background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

results: of the 50 samples tested by the elisa method, 26 samples (52%) were positive for igg . no samples were positive for igm. of the 50 samples tested by the chemiluminescence method, 28 samples (56%) were positive for igg. no samples were positive for igm. conclusions: a significant relationship between the age of the youngest child and the infection rate was seen. no significant correlati...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...

universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (dtwp) has been applied in iran for almost 50 years. periodic assessment of immunogenicity of this vaccine is an important aspect of successful m...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...

background toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. objectives the purpose of this study was to identify and determine the prevalence of toxoplasma gondii in clinical samples collected from patients ...

background: aflatoxin m1 (afm1) as a carcinogenic and toxic compound has considerable significance from public health viewpoint. the aim of this study was to determine the level of afm1 in pasteurized and uht milk in west-azerbaijan province of iran. methods: a total of 360 commercial milk samples including 220 pasteurized milk and 140 ultra-high-temperature (uht) milk were purchased randomly f...