The immobilization of biological molecules onto polymeric membranes to produce biofunctional membranes is used for selective catalysis, separation, analysis, and artificial organs. Normally, random immobilization of enzymes onto polymeric membranes leads to dramatic reduction in activity due to chemical reactions involved in enzyme immobilization, multiple-point binding, etc., and the extent of...

Enzymes have several excellent catalytic features, and the last few years seen a revolution in biocatalysis, which has grown from using one enzyme to multiple enzymes cascade reactions, where product of reaction is substrate for subsequent one. However, stability remains an issue despite many benefits system. When are exposed harsh process conditions, deactivation occurs, changes activity enzym...

Microbial intradiol dioxygenases have been shown to have a great potential for bioremediation; however, their structure is sensitive to various environmental and chemical agents. Immobilization techniques allow for the improvement of enzyme properties. This is the first report on use of glyoxyl agarose and calcium alginate as matrixes for the immobilization of protocatechuate 3,4-dioxygenase. M...

A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. The significant factors influencing the immobilization process s...

Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on nonc...

Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, has led to important applications for biosensors, biofuel cells, bioelectrocatalysis. Enzyme immobilization is basis bioelectrochemistry, as itself determines enzyme/material interface thus electrochemical performance. Amongst range methods enzyme immobilization, one-step approaches feature rap...