Lysosomal enzyme release from PMN exposed to STZ was examined using PMN from normal and CGD donors. Normal PMN showed an increase of extracellular lysosomal enzyme activity and a marked reduction of totol (extra-plus intra-cellular) enzyme activity after phagocytosis. On the other hand, in PMN from CGD patients, such a reduction of total enzyme activity was not observed and much more enzyme tha...

We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine d...

The simplest transducer in the development of potentiometric biosensors is the glass pH electrode. Glucose oxidase immobilization was achieved using cellophane [1], nylon [1-6] or nitrocellulose [5,7] membranes that are subsequently fixed on the sensitive bulb of the pH electrode that senses the pH diminution, as a result of the biocatalytical reaction occurring in the enzyme layer (glucose oxi...

Glucose oxidase has wide applications in the pharmaceutical, chemical, and food industries. Many recent studies have enhanced key properties of this enzyme using directed evolution, yet without being able to reveal why these mutations are actually beneficial. This work presents a synergistic combination of experimental and computational methods, indicating how mutations, even when distant from ...

This review paper aimed to provides precious information about the function and use of different enzymes in dairy food applications. An enzyme is called a protein and catalyzes a specific reaction. Every enzyme is intended to initiate a particular reaction with a specific outcome. Moreover, numerous enzymes are present in the human body. Dairy food applications include the use of different enzy...

Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose-controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built glucose oxidase-polymer hybrids that assemble in presence an organometallic catalyst: hemin. The design hybrid is optimized to increase effectiveness directional channeling at low substrate concentration. Importantly...

The apparent Michaelis constant, K(M), for glutamate oxidase (GluOx) immobilised on Pt electrodes increased systematically with enzyme loading. The effect was due, at least in part, to electrostatic repulsion between neighbouring oxidase molecules and the anionic substrate, glutamate (Glu). This understanding has allowed us to increase the Glu sensitivity of GluOx-based amperometric biosensors ...

The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure by precipitation with ammonium sulfate at a saturation rate 70% specific activity in this step reached (10.802) units/mg fold 1.276, was followed dialysis step, 13,593 48.184. Finally, ion exchange chromatography using DEAE-Cellulose column, 19.355 units/mg, 36.59, after which migrate...

Objectives. Using apocynin (inhibitor of NADPH oxidase), and Mitoquinol 10 nitrate (MitoQ; mitochondrial-targeted antioxidant), we addressed the importance of mitochondria versus NADPH oxidase-derived ROS in glucose-induced apoptosis of pericytes. Methods. NADPH oxidase was localised using Western blot analysis and cytochrome C reduction assay. Apoptosis was detected by measuring caspase-3 acti...