Parthenium sp. is a noxious weed which threatens the environment and biodiversity due to its rapid invasion. This lignocellulosic weed was investigated for its potential in biofuel production by subjecting it to mild alkali pretreatment followed by enzymatic saccharification which resulted in significant amount of fermentable sugar yield (76.6%). Optimization of enzymatic hydrolysis variables s...

Decreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomass samples having different lignin contents and investigated biomass saccharification efficiency. ...

HIGHLIGHTS C. cubensis-P. pinophilum enzyme blend resulted in high levels of synergy Mild hydrothermal pretreatment reduced the hemicellulases requirement hydrolysis The best results were obtained by enzymatic H60-SCB saccharification

Cellulose is one of the most abundant non-degradable organic compound on earth. Near about half of the municipal and agricultural solid wastes contain cellulose or their derivatives. Saccharification or enzymatic hydrolysis of cellulosic wastes liberates glucose. In the present work, cellulase from Cellulomonas uda was extracted, partially purified by dialysis and immobilized on an organic supp...

The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation using Trichoderma viride. Enzymatic activity of the cellulosic complex was measured during saccharification of substrates filter paper, eucalyptus sawdust, and corncob, and compared...

Kitchen wastes containing high amounts of carbohydrates have potential as low-cost substrates for fermentable sugar production. In this study, enzymatic saccharification of kitchen waste was carried out. Response surface methodology (RSM) was applied to optimize the enzymatic saccharification conditions of kitchen waste. This paper presents analysis of RSM in a predictive model of the combined ...

To optimize the enzymatic conversion of widely available lignocellulosic biomass-wheat straw (WS), pretreatment facilitating the enzymatic saccharification process was first performed by alkaline peroxide, resulting in a substrate consisting of 60.17% cellulose, 29.53% hemicelluloses, and 4.59% lignin. Using response surface methodology, the combined effects of enzyme loading, substrate concent...

BACKGROUND The development of affordable woody biomass feedstocks represents a significant opportunity in the development of cellulosic biofuels. Primary woodchips produced by forest mills are considered an ideal feedstock, but the prices they command on the market are currently too expensive for biorefineries. In comparison, forestry residues represent a potential low-cost input but are consid...

This study investigated the degradation and enzymatic saccharification of cornstalk by white-rot and brown rot fungi. The fungal strains Trametes pubescens Cui 7571, Trametes velutina Dai 10149, and Antrodia wangii Cui 7568 were analyzed in solid-state fermentation cultures. Various extracellular enzyme activities were assessed to determine biochemical changes during the degradation process. Fo...

The major objective of the present investigation was to evaluate the effect of enzymatic pretreatment on Lantana camara for improved yield of reducing sugar and bioethanol production. An optimum enzymatic delignification (88.79 %) was achieved after 8 h of incubation. After delignification the substrate was further treated with the mixture of carbohydratases for appropriate saccharification. Th...