Bacillus cereus strains were tested for production of diarrheal enterotoxin by the reverse passive latex agglutination test and for presence of B. cereus enterotoxin gene (bceT) by polymerase chain reaction. About 50% of 56 B. cereus strains reacted positive in broth culture in the reverse passive latex agglutination test, while the bceT gene was detected in 41.1%. Sixteen percent of the strain...

Rapid and sensitive method for detection of Staphylococcus aureus enterotoxin genes in milk sampleMahantesh M Kurjogi, Basappa B Kaliwal

We used Salmonella enteritidis serotype dublin strain SL1438, a nonreverting, aromatic-dependent, histidine-requiring mutant, as a recipient for a recombinant plasmid coding for production of the nontoxic B subunit of the heat-labile Escherichia coli enterotoxin. The S. enteritidis derivative EL23 produced heat-labile enterotoxin subunit B that was indistinguishable from heat-labile enterotoxin...

The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C1, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude ...

OBJECTIVE The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. MATERIALS AND METHODS In this experimentally study, 300 Staphylococcus aureus (S. aureus) strains isolated from clinical samples...

The in vitro exposure of staphylococcal enterotoxin B to trypsin resulted in the formation within 30 min of a product (enterotoxin-T) unchanged in molecular weight, but with threonine as a second NH2 terminus. Upon reduction of the -SSbridge in enterotoxin-T, two fragments were separated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The peptide bond between Lys-97 and Thr-98 ...

The in vitro exposure of staphylococcal enterotoxin B to trypsin resulted in the formation within 30 min of a product (enterotoxin-T) unchanged in molecular weight, but with threonine as a second NH2 terminus. Upon reduction of the -SSbridge in enterotoxin-T, two fragments were separated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The peptide bond between Lys-97 and Thr-98 ...

Culture supernatants of 30 enterotoxin-producing Bacillus cereus isolates produced a characteristic progressive destruction of McCoy cell monolayers. Enterotoxin-negative B. cereus and other group 1 Bacillus spp. caused no monolayer disruption. The McCoy cell tissue culture system appears to provide a rapid screening assay for detection of enterotoxin-producing B. cereus.

Out of 321 strains of Bacillus cereus from several sources and isolated in four different countries, 239 (74%) produced cytotoxins. Only 127 (53%) of the cytotoxic strains were positive for the B-component gene of the haemolysin BL (enterotoxin) by polymerase chain reaction (PCR). Western blots using antiserum produced against enterotoxin(s) gave positive results for 199 (83%) of the cytotoxic ...

conclusions the results revealed, the specific primers that amplified the ente gene were able to amplify the staphylococcal enterotoxin-like toxin type p gene. the specific primers for the entp gene were amplified a fragmented gene (700 bp) showed 100% homology with entp reference gene and also 80% homology with enta and ente genes. results the pcr amplification method was optimized for entp ge...