background: recently, embryonic stem (es) cells have become very important resources in basic medical researches. these cells can differetiate into derivatives of all primary germ layers. objectives: in order to isolate embryonic stem cells in vitro, the blastocyst were cultured and the morphological aspects, population doubling time, alkalin phosphatse and differentiation properties of the cel...

objective: it is hypothesized that stem cells have the capability to form tumors after transplantation. spermatogonial stem cells have proliferation potency and colonization ability related to express pluripotency genes such as c-myc. the primary aim of this study is to investigate tumorigenicity ability of these cells after in vitro cultivation and inoculation in athymic animals. methods: sper...

Objective: The depletion of platelets in patients under different clinical conditions like thrombocytopenia is often associated with a significant mortality factor. Alternative modes platelet propagation from other resources, including stem cells, are evaluated vitro conditions. Embryonic (ES) cells can proliferate and differentiate into various cell types. This study the generation functional ...

the way to overcome current limitations in the generation of glucose-responsive insulin-producing cells is selective enrichment of the number of definitive endoderm (de) progenitor cells. sox17 is the marker of mesendoderm and definitive endoderm. the aim of the present research was to study the potential of sox17 knock-in cgr8 mouse embryonic stem (es) cells to differentiate into insulin produ...

background: the transcription factor oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. establishment of an oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. methods: in the present study, we report construction of a recombin...

introduction: considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with p19 cells on the line of differentiation. materials and methods: for this purpose, p19 cells were cultured directly in semisolid medium. these cells proliferated and primarily differentiated to colonie...

background: the present study was designed to evaluate the homing potential of mouse embryonic stem cells (esc) treated with erythropoietin (epo) in hematopoietic organs such as spleen and liver after transplantation using morphological and immuno-histochemical techniques. methods: day-four embryoid body (eb)-derived cells were dissociated and re-plated in medium in the presence and absence of ...

stem cells are the cells having some distinguishing characteristics like longevity, high capacity of self-renewal and differentiation, quiescence and highly error-free proliferation. almost all stem cells have the potential of lineage reprogramming, i.e. inter-conversion of cell lineages. they also have the potential to differentiate into almost all kinds of cells. these cells have been found i...