Neurons are multifaceted cells that carry information essential for a variety of functions including sensation, motor movement, learning, and memory. Studying neurons in vivo can be challenging due to their complexity, their varied and dynamic environments, and technical limitations. For these reasons, studying neurons in vitro can prove beneficial to unravel the complex mysteries of neurons. T...

T H E discovery of the organization centre of Amphibia by Spemann (1918) opened a new field in the outlook of experimental embryology; but there were several technical difficulties to be overcome in the application of the concept of the organizer to avian embryology. In this connexion the tissue culture technique has several advantages over the earlier methods of in situ sectioning or chorio-al...

the major objective of seedless grape breeding program is production of grapes with high quality. breeding of seedless grapes is difficult because the embryos are aborted after fertilization. embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. in the present study, the effect of paternal genotypes and different iaa concentrations in presence of ba appli...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

The embryo culture technique has been improving, but the detailed demands for energy substrates such as glucose, fructose, pyruvate and lactate of preimplantation embryos are still unclear. In the present study, the demands of pig preimplantation embryos at each different developmental stage were investigated by use of parthenogenetic diploids as a model of pig preimplantation embryos. Pig part...

diminished ovarian reserve (dor) significantly decreases the success rate of the assisted reproductive technique (art). in this study, we assessed the effect of melatonin on the art outcomes in women with dor. a double-blinded, randomized, clinical trial was performed on 80 women with dor as a pilot study in shiraz, between 2014 and 2015. dor was defined as the presence of 2 of the following 3 ...

the success rate of embryo production critically depends on several factors. one of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (ivf) and in vitro embryo culture (ivc) (1, 2). besides the technique selected for ivf / in vitro embryo production (ivep), there is an important consideration called ivf/ivep culture dishes disinfection (3). for many years...

taxus chinensis var. mairei is a rare and endangered medicinal plant species distributed in china. in order to promote fast propagation and preserve the natural resources, conditions for in vitro germination and seedling development of embryos of t. chinensis var. mairei from anhui or zhejiang were investigated. results showed that in vitro germination rate of excised embryos cultured under 14 ...

The success rate of embryo production critically depends on several factors. One of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (IVF) and in vitro embryo culture (IVC) (1, 2). Besides the technique selected for IVF / in vitro embryo production (IVEP), there is an important consideration called IVF/IVEP culture dishes disin...