The Laser-nephelometric technique is characterized by its easy handling, accuracy, good reproducibility and especially by the short time in which reliable results are available. A very good correlation was found between the results obtained by the electroimmunoassay and those obtained by Laser-nephelometry. In both techniques alpha2-macroglobulin is the largest serum protein that can be determi...

The presence of apolipoproteins A-I, E, C-II, and C-III and the absence of apolipoprotein B was demonstrated in human cerebrospinal fluid. The concentration of apolipoproteins was measured by electroimmunoassay. Apolipoproteins E, C-II, and C-III were present in cerebrospinal fluid at 3--5% of their concentration in plasma; the cerebrospinal fluid level of apolipoprotein A-I was 0.4%. Most of t...

The measurement of microalbuminuria in diabetics is now clinically important as a predictor of later overt clinical proteinuria, reflecting advanced diabetic nephropathy [l-3]. Urine albumin assays suitable for routine use in the clinical laboratory are therefore important. Measurement of microalbuminuria by current techniques including immunodiffusion [4] and electroimmunoassay [5] are time-co...

An original competitive enzyme inhibition immunoassay has been developed for determination of total apolipoprotein A-I (apo A-I) in serum and in plasma. This specific assay involves a single monoclonal antibody (F59 4A12 2F4, directed to the -COOH terminal region of the apo A-I molecule) and a stable secondary plasma standard. Delipidation of serum samples exposed no additional antigenic sites,...

We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those ...

Two new commercial assays for the detection of degradation products of fibrinogen/fibrin (FDP) were evaluated against two standard procedures. The first, a new hemagglutination inhibition (HAI) assay using glutaraldehyde-treated cells, was compared with the tanned red cell hemagglutination inhibition immunoassay (TRCHII). Analysis of 43 samples from patients with a variety of bleeding disorders...

Activation of C3 by nylon 6.6 and polymethyl methacrylate (PMMA) polymers in whole canine serum was investigated using Laurell electroimmunoassay and antigen-antibody crossed immunoelectrophoresis (XAA). Canine sera were utilized because most artificial blood contact materials used in prosthetic devices are tested in dogs. The measurements were made possible by converting the polymers to microp...