The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region were then deleted by site-directed mutagenesis. The translation of regions corresponding to the cell-wall- and membrane-binding domains was prevented by introduction of stop codons upstream of these do...

Protein G, the immunoglobin G-binding surface protein of group C and G streptococci, also binds serum albumin. The albumin-binding site on protein G is distinct from the immunoglobulin G-binding site. By mild acid hydrolysis of the papain-liberated protein G fragment (35 kDa), a 28-kDa fragment was produced which retained full immunoglobulin G-binding activity (determined by Scatchard plotting)...

PURPOSE The purpose of this study was to evaluate the psychometric quality and feasibility of measurements for screening dysphagia in older adults to identify the 'right tool' for nurses to use in nursing homes. METHODS A systematic review was done. Electronic databases were searched for studies related to dysphagia screening measurements. A checklist was used to evaluate the psychometric qua...

Anne Monceaux, Ambjörn Naeve, Miguel-Angel Sicilia, Elena GarciaBarriocanal, Sinuhé Arroyo and Joanna Guss EADS-Innovation Works,18, rue Marius TERCE, Toulouse, France – [email protected] Royal Institute of Technology (KTH), Stockholm & University of Uppsala. Uppsala Learning Lab (ULL), Kyrkogårdsgatan 2 C Uppsala, Sweden;– [email protected] University of Alcalá de Henares. Polytechnical Bui...

Background: Dysphagia is one of the major post-stroke complications that can severely damage patient's quality life and even endangers patients’ lives. Shaker exercise indirect methods, behavioral swallowing techniques, may increase speed sensitivity. The aim study was to evaluate effect Shaker exercise on dysphagia level among patients with cerebral vascular stroke. Study design: A quasi-...

Differentiation and morphogenetic potential of Allium commutatum Guss. callus tissue was investigated in five subcultures. Callus tissue was derived from root tips of in vitro grown seedlings and maintained on MS medium supplemented with 3% sucrose, 0.8% agar, 4.5 pM 2,4-D and 4.6 pM kinetin. Pieces of calli were subcultured on media with different concentrations and combinations of 2,4-D (1.0,...