Equal channel angular rolling (ECAR) process is one of the severe plastic deformation (SPD) methods that have been used to make ultra-fine grain (UFG) materials with improving the mechanical properties of samples. After performing the process at several paths, a large strain is applied to the material that can cause decreasing the grain size and improving the mechanical and physical properties ...

equal channel angular rolling (ecar) process is one of the methods that have been used to makeultra-fine materials by imposing severe plastic deformation. after repeating the process severaltimes, a large effective strain is applied to the sample that can cause decreasing the grain size andimproving the mechanical and physical properties of the metal. in this study, thermal conductivityof the s...

Air-liquid interface cell culture is an organotypic model for study of differentiated functional airway epithelium in vitro. Dysregulation of cellular energy metabolism and mitochondrial function have been suggested to contribute to airway diseases. However, there is currently no established method to determine oxygen consumption and glycolysis in airway epithelium in air-liquid interface. In o...

Background: Endometriosis is a painful disorder characterized by the growth of endometrial tissue outside uterine cavity. Here, we investigated effects cinnamic acid isolated from Chinese medicinal plant Cinnamomum cassia Presl on primary stromal cells. Methods: Immunohistochemistry was used to examine protein expression and cell purity. Quantitative RT-PCR conducted assess mRNA expression, Wes...

Purpose To investigate whether high glucose (HG) induces mitochondrial dysfunction and promotes apoptosis in retinal Müller cells. Methods Rat retinal Müller cells (rMC-1) grown in normal (N) or HG (30 mM glucose) medium for 7 days were subjected to MitoTracker Red staining to identify the mitochondrial network. Digital images of mitochondria were captured in live cells under confocal microsc...

Transforming growth factor (TGF)-β has been associated with podocyte injury; we have examined its effect on podocyte bioenergetics. We studied transformed mouse podocytes, exposed to TGF-β1, using a label-free assay system, Seahorse XF24, which measures oxygen consumption rates (OCR) and extracellular acidification rates (ECAR). Both basal OCR and ATP generation-coupled OCR were significantly h...

N,N-Dimethylformamide (DMF) affects cellular differentiation, causes hepatotoxicity and gastric irritation, and may be carcinogenic. Since these processes involve changes in cellular pH homeostasis, we investigated the effects of DMF on H+ extrusion and cytosolic pH (pHi) of mouse hepatoma cells (Hepa 1C1C7). Extracellular pH was monitored using a silicon-based sensor system (Cytosensor microph...

Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust growth resist death. MicroRNAs (miRNAs) have been known play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs experimentally confirmed as critical regulators of NSCLC. TCGA datasets were analyzed screen for differential...

Genetically engineered T lymphocytes are a promising option for cancer therapy. Prior to adoptive transfer they have to be expanded in vitro to reach therapeutically sufficient numbers. So far, no universal method exists for selective in vitro expansion of engineered T lymphocytes. In order to overcome this problem and for proof of concept we incorporated a novel unique peptide sequence of ten ...