The aim of the present study is to determine the characteristics of genotype and phenotype of Echinococcus granulosus derived from wild sheep and to compare them with the strains of E. granulosus sensu stricto (sheep-dog) and E. granulosus camel strain (camel-dog) in Iran. In Khojir National Park, near Tehran, Iran, a fertile hydatid cyst was recently found in the liver of a dead wild sheep (Ov...

b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected w...

Cystic Echinococcosis is an important zoonosis in the sheep rising areas of Iran. To develop a simple and reliable diagnostic method for Echinococcus infection in definitive hosts, E. granulosus polyoclonal antibodies (PolyAbs) were prepared from adult worm in rabbit. A selected PoAb was used for coproantigen detection in faecal samples obtained from animals naturally infected with Echinococcus...

Cystic echinococcosis (CE) is a cosmopolitan disease caused by the dog tapeworm Echinococcus granulosus. The disease is difficult to diagnose, treat, and control and is responsible for considerable human morbidity and mortality globally. There is an urgent need for new diagnostic tests and new drugs for treatment of CE and the development of a vaccine against adult worms of E. granulosus in dog...

The larval stage of Echinococcus granulosus causes cystic echinococcosis, a neglected infectious disease that constitutes a major public health problem in developing countries. Despite being under constant barrage by the immune system, E. granulosus modulates antiparasite immune responses and persists in the human hosts with detectable humoral and cellular responses against the parasite. In vit...

echinococcosis is one of the world’s relevant zoonoses, affecting both humans and their domestic animals and caused by infection with the metacestode stage of the dog tapeworm, echinococcus granulosus. in the present study, twenty mice were randomly divided into 4 groups of 5. each mouse in groups 1, 2 and 3 was immunized with 100 μg of hydatid cyst fluid protein, protoscolex and adult worms of...

BACKGROUND/AIM To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. MATERIALS AND METHODS DNA was isolated...

Prevalence of Echinococcus granulosus genotype G1 was investigated in 55 cattle, 129 buffaloes, 10 sheep and 10 goat samples by polymerase chain reaction (PCR). These 204 samples were collected from 64 liver and 140 lungs of slaughtered animals from three districts Okara (n= 29), Jhang (n = 43) and Lahore (n= 132). E. granulosus G1 genomic DNA was used for specific amplification of a 254-bp fra...

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance in China. Our main objective was to discuss the genotypes and genetic diversity of E. granulosus present in domestic animals and humans in western China. A total of 45 hydatid cyst samples were collected from sheep, humans, and a yak and subjected to an analysis of the sequences of mit...

The identification of the genotypes of Echinococcus granulosus present in livestock and wild animals within regions endemic for cystic echinococcosis (CE) is epidemiologically important. Individual strains display different biological characteristics that contribute to outbreaks of CE and that must be taken into account in the design of intervention programs. In this study, samples of hydatid c...