background and objective: escherichia coli o157:h7, an emerging pathogen, causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. the goal of this study was to evaluate the conjugate of e. coli o157: h7 lipopolysaccharide (lps) with diphtheria toxoid (dt) as a candidate vaccine in mice model. material and methods: lps from e. coli o157:h7 was extracted by hot ...

Recent water related outbreaks of shiga toxin producing E. coli O157 have resulted in increased attention of the water industry to this potentially deadly pathogen. Current methods to detect E. coli O157 and its virulence genes are laborious and time-consuming. Specificity, sensitivity and simple use of a real-time PCR method makes it an attractive alternative for the detection of STEC E. coli ...

background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...

This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19 significantly reduced E. coli O157:H7 bacterial load in vitro in a multiplicity of infection (MOI...

Controlling the prevalence of Escherichia coli O157 in cattle at the pre-harvest level is critical to reduce outbreaks of this pathogen in humans. Multilayers of factors including the environmental and bacterial factors modulate the colonization and persistence of E. coli O157 in cattle that serve as a reservoir of this pathogen. Here, we report animal factors contributing to the prevalence of ...

The use of a minimal lactose enrichment broth (MLB) in conjunction with immunomagnetic electrochemiluminescence detection (IM-ECL) was evaluated for the estimation of viable Escherichia coli O157 populations in surface water samples. In principle, E. coli O157 populations (C(initial E. coli O157)) can be derived from enrichment data according to the equation: C(initial E. coli O157) = C(initial...

to determine the contamination of traditional cheeses to e. coli o157:h7 this study was conducted in chaharmahal and bakhtiari province. two hundred cheeses samples were obtained randomly and bacteriological examinations were done on all isolates, by culture in selective enrichment media and after that, selective plating. suspected colonies were examined with o and h antisera for identification...

Escherichia coli O157:H7 is a serious and common human pathogen that can cause diarrhoea, haemorrhagic colitis, and haemolytic uraemic syndrome (HUS). This study evaluated the enrichment, detection and confirmation procedures for the isolation of E. coli O157:H7 from raw ground beef and raw drinking milk. The purpose of this investigation was to compare Rainbow Agar O157 (RB; Biolog, Hayward, U...

Background: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing human infections. These infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. The first intimate bacterial adhesion, intimin (I), with its own receptor translocated intimin receptor (Tir) and E. coli secreted protein...