the raini cashmere goat is one of the iranian goat breeds with a high economic value on international markets due to its high production and high quality. the bmp15 gene regulates granulose cell proliferation and differentiation by promoting granulosa cell mitosis, suppressing follicle-stimulating hormone receptor expression, and stimulating the kit ligand expression. this gene plays a pivotal ...

We developed and validated two species-specific qPCR markers to detect American Eel environmental DNA. Marker validation included assay design, specificity sensitivity testing, in vivo laboratory field experiments. Markers AME1 AME2 targeted 116 129 bp fragments of the mitochondrial NADH dehydrogenase subunit 2 cytochrome b genes, respectively. were 94%–100% homologous for all 49 aligned sequen...

this study was conducted to detect polymorphism of gene bmp15, a member of transforming growthfactor β (tgfβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. samples were randomly selected from 91 najdi goats in 3 geographicallocations, northwest, southeast and center of khuzestan province. after dna extraction, amplification of235 ...

During the last few decades, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant biotechnology and their genetics studies. There are different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiates in two types first non PCR based (RFLP) and second is PCR based m...

BACKGROUND The main goal of the work was to analyse intraspecific variation in Elettaria cardamomum Maton (cardamom) using genome size, cytological studies and molecular marker data. Nuclear DNA content and molecular marker details furnish data on genome size and genetic diversity respectively among the studied accessions and both complement each other for evolutionary and taxonomic studies. ...

W12E keratinocytes maintaining episomal copies of HPV DNA were separated according to size by centrifugal elutriation. HPV DNA copy number was greatly increased in the largest, most differentiated cells of the population. The large cells with the highest HPV copy number also showed evidence of endoreduplication of host cell DNA. Other cell lines maintaining episomal copies of HPV18 and HPV31 we...

Objective: Berberis vulgaris contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of B. vulgaris on DNA cleavage. <span style="font-size: medium;"...

Fluorescently labeled size standards are indispensable for genotyping via an automated DNA sequencer. Microsatellites, amplified fragment-length polymorphisms (AFLPs), single nucleotide polymorphisms (SNPs), and random amplified polymorphic DNA (RAPD) can all be assayed on platforms such as the ABI Prism® 377 DNA Sequencer or the ABI Prism 3100-Avant Genetic Analyzer (both from Applied Biosyste...

DNA marker is commonly used to determine the size of fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure prepare recombinant plasmids pSY-60 which was partially digested one restriction enzyme for generating markers 7 from 60 420 bp. The included synthesis bp fragment with EcoRI sites at both ends using PCR extension, self-ligation an...