This chapter reviews recent advances in the understanding of DNA release dynamics from polymeric gene delivery systems. Atomic force microscopy (AFM) has recently been applied to monitor DNA release from bioreducible polyplex nanoparticles and layer-by-layer (LbL) thin films in real time. DNA release dynamics is central to the understanding and control of gene delivery from gene delivery nano-s...

DNA nanostructures are emerging as a versatile platform for controlled drug delivery result of recent progress in production yield and strategies to obtain prolonged stability biological environments. The construction from this unique biomaterial provides unparalleled control over structural functional parameters. Recent applications origami-based nanocarriers therapeutic preclinical phases hig...

BACKGROUND Particulates incorporating DNA are promising vehicles for gene delivery, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Zein, a hydrophobic protein from corn, is biocompatible and has properties that make it a promising candidate material for particulate delivery, including its ability to form nanospheres through coacerv...

We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs otherwise non-interacting proteins. The is designed to recognize bind a specific antibody and, in response, undergo conformational change leads release DNA strand, termed “translator,” regulates activity downstream target protein. As proof principle, we demons...

We report the design of erodible 'mixed multilayer' coatings fabricated using plasmid DNA and combinations of both hydrolytically degradable and charge-shifting cationic polymer building blocks. Films fabricated layer-by-layer using combinations of a model poly(β-amino ester) (polymer 1) and a model charge-shifting polymer (polymer 2) exhibited DNA release profiles that were substantially diffe...