background hepatitis b virus (hbv) covalently closed circular dna (cccdna) served as a vital role in the life cycle of the virus and persistent infection. however, specific and quantitative methods for cccdna detection have not been available. objectives our aim was to develop and primarily evaluate a quantitative method for hbv cccdna based on magnetic capture hybridization and quantitative pc...

to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...

This study was conducted to determine the location of DNA segment with homology to the rat conserved genomic DNA in human chromosomes. The labeled rat genomic DNA was hybridized with normal human (male) metaphases. The study of 74 metaphases after fluorescence in situ hybridization showed 371 twin-spot signals on human chromosomes. Statistical analysis indicated that the specific accumulation o...

Surface hybridization reactions, in which sequence-specific recognition occurs between immobilized and solution nucleic acids, are routinely carried out to quantify and interpret genomic information. Although hybridization is fairly well understood in bulk solution, the greater complexity of an interfacial environment presents new challenges to a fundamental understanding, and hence application...

we have analyzed paraffin sections from 55 patients with histologically confirmed hodgkin’s disease (hd) for the presence of epstein-barr virus (ebv) markers using in situ hybridization to detect the ebv-encoded rnas (ebers) and immunohistochemistry to identify latent membrane protein-1 (lmp1) expression. tissue specimens from 55 cases of hodgkin’s disease included 22-mixed cellularity (mc), 27...