aim : the aim of this survey was to compare four dna extraction methods from iranian sheep strain e.granulosus isolates. background : cystic echinococcosis (ce) caused by the metacestode of the dog tapeworm echinococcus spp., is a global zoonotic infection which is economically important and constitutes a major threat to public health in many countries. strains characterization is essential for...

Extraction of high-quality and-quantity DNA is a fundamental requirement for genetic research. It is very important to address the use of DNA extraction methods that are simple and cost-effective in gene polymorphism with large number of samples. This study was designed to investigate the optimal DNA extraction from lymphocytic cells by salt-out method. In this study, 200 blood samples of the t...

background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...

dna extraction with an appropriate quality and quantity is the first step in molecular identification of some group of insects that their identification morphologically is difficult and dependent on a particular life stage, size or gender. selection of a suitable protocol is a key factor to provide a pure dna. in this study quality and quantity of five extraction methods used in entomological l...

background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

the dna molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. understanding how drug molecules interact with dna has become an active research area in the interface between chemistry, molecular biology and medicine. dna extraction has been suggested as a main step affecting molecular dna technology such as pcr and pcr-based methods. therefor...

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...