A new method to determine microbial (bacterial and fungal) activity in various freshwater habitats is described. Based on microbial reduction of dimethyl sulfoxide (DMSO) to dimethyl sulfide (DMS), our DMSO reduction method allows measurement of the respiratory activity in interstitial water, as well as in the water column. DMSO is added to water samples at a concentration (0.75% [vol/vol] or 1...

OBJECTIVE To examine the feasibility of dimethyl sulfoxide (DMSO) incorporation into relatively hydrophilic resins as a new potential method to improve the durability of resin-dentin bonds. METHODS Six experimental light-curing BisGMA/HEMA resins solvated in ethanol and DMSO with increasing concentrations of DMSO (0, 0.5, 1, 2, 4 and 10wt%) were prepared. The degree of conversion (DC) was eva...

Understanding the effects of organic co-solvents on protein structure and function is pivotal to engineering enzymes for biotransformation in non-aqueous solvents. The effects of DMSO on the catalytic activity of cytochrome P450 BM3 have previously been investigated and the importance of Phe87 in its organic co-solvent tolerance was identified. To probe the DMSO inactivation mechanism and the f...

cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...

1H NMR spectroscopy was used to monitor the reactions of an arylidene 2-thiohydantoin derivative, 3-((phenylmethylene)amino)-2-thioxo-4- imidazolidinone (3), with PdCl2, cis-[PdCl2(dmso-S)2] and K2[PdCl4] in DMSO-d6 order elucidate reaction kinetics mechanism. The derivative 3 formed cis-[Pd(3-N,S)(dmso-S)2]+ complex (5) PdCl2 cis-[PdCl2(dmso-S)2], while no observed. A two-step mechanism for ci...

The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocytes using vitrification. The vitrification of sheep oocytes was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more...

Proton translocation coupled to dimethyl sulfoxide (DMSO) reduction was examined in Escherichia coli HB101 grown anaerobically on glycerol and DMSO. Rapid acidification of the medium was observed when an anaerobic suspension of cells, preincubated with glycerol, was pulsed with DMSO, methionine sulfoxide, nitrate, or trimethylamine N-oxide. The DMSO-induced acidification was sensitive to the un...

Dimethyl sulfoxide (DMSO) is widely used in biological studies as a cryoprotective agent for cells and tissues, and also for cryopreserved platelets (PLTs). However, few data on the toxic effects of DMSO following intravenous infusion of cryopreserved PLTs are available. The aim of this study was to explore dose-related effects of DMSO on red blood cells (RBCs), PLTs and vascular endothelial ce...

BACKGROUND The survival of autologous fat graft tissue is dependent on various factors, such as vascularization and inflammation. OBJECTIVE This study aims to evaluate the possible beneficial effects of dimethylsulfoxide (DMSO) on fat graft volume and survival. METHODS Eighteen male Wistar albino rats were divided randomly into three groups. An autologous fat graft obtained from the inguina...

DMSO is used as a treatment for interstitial cystitis and this study examined the effects of luminal DMSO treatment on bladder function and histology. Porcine bladder was incubated without (controls) or with DMSO (50%) applied to the luminal surface and the release of ATP, acetylcholine, and LDH assessed during incubation and in tissues strips after DMSO incubation. Luminally applied DMSO cause...