It is shown by tracer experiments with DL-[2-3H,5-14C]- and DL-[(RS)-5-3H,5-14C]rnithine, that the metabolic conversion of ornithine into proline, in three plant species (Nicotiana tabacum, Datura stramonium, and Lupinus angustifolius), takes place with maintenance of the delta-hydrogen atoms but with loss of the alpha-hydrogen atom. This indicates a route via alpha-keto-delta-aminovaleric acid...

Materials. DL-Methionine-l-'4C (1.41 and 3.54 mCi/mmole), and DL-ornithine-l -†̃ †̃@ C (3.3 1 mCi/mmole) were obtained from New England Nuclear Corporation, Boston, Mass. The hydrochloride salts of putrescine, spermidine, and spermine and Tns, glutathione, and AlP were obtained from Sigma Chemical Company, St. Louis, Mo. S-Adenosyl-L-methionine and pyridoxal phosphate were obtained from Calbi...

Eflornithine is not official in any of the pharmacopoeias, the literarture survey did not reveal any analytical method for quantitative estimation of eflornithine. Thus there is a need for the development of newer effective, sensitive, accurate and economical methods of analysis for quantitative estimations of eflornithine as an active pharmaceutical ingredient. The aim of this work was to deve...

The present study was conducted in a one-factorial arrangement to determine the effects of dl-2-hydroxy-4-methylthiobutyrate (dl-HMTB) on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability. Barrows (n 6; aged 35 d; weight 8.6 kg), implanted with arterial, portal, mesenteric and gastric catheters, were fed a diet containing dl-methionine (dl-MET) or ...

Milk urea nitrogen (MUN) and blood are correlated with balance excretion; however, there is also a genetic component to MUN concentrations that could be associated differences in transport. It was hypothesized portion of the variation among cows caused by gastrointestinal kidney clearance rates. Eight lactating varying while fed common diet were infused [15N15N]urea determine N entry rate (UER)...

Trypsin catalyses the hydrolysis of suitable derivatives of basic amino acids including peptides, amides and esters. Esters are the most sensitive substrates, and it was decided to use them in the present study, which had as one of its aims the elucidation of the relationship between the length of the side chain and susceptibility to hydrolysis by trypsin. Previous investigations had shown that...