Drug-induced immune thrombocytopenia (DITP) is caused by drug-dependent antibodies (DDAbs) that are nonreactive in themselves but bind tightly to specific platelet membrane glycoproteins (GP) when soluble drug is present at pharmacologic concentrations. This reaction takes place without covalent linkage of drug to the target, indicating that drug does not function as a classical hapten to promo...

The hyperthermophilic bacterium Thermotoga maritima has a noncanonical nucleoside triphosphatase that catalyzes the conversion of inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP) into inosine monophosphate (IMP), deoxyinosine monophosphate (IMP) and xanthosine monophosphate (XMP), respectively. The k(cat)/K(m) values determined at 323 and 353 K fall...

Deamination of nucleobases in DNA and RNA results in the formation of xanthine (X), hypoxanthine (I), oxanine, and uracil, all of which are miscoding and mutagenic in DNA and can interfere with RNA editing and function. Among many forms of nucleic acid damage, deamination arises from several unrelated mechanisms, including hydrolysis, nitrosative chemistry, and deaminase enzymes. Here we presen...

Nucleoside triphosphate pyrophosphohydrolase has been isolated from rabbit reticulocytes. The enzyme has been pursed over 2000-fold from the cell lysate. Isolation and analysis procedures are described. The enzyme has been shown to catalyze the hydrolysis of ITP to yield IMP and PPi. Other substrates are dITP, XTP, and, to a lesser extent GTP, dGTP, UTP, and dTJTP. ATP, dATP, CTP, and dCTP are ...

Mammalian inosine triphosphatase encoded by ITPA gene hydrolyzes ITP and dITP to monophosphates, avoiding their deleterious effects. Itpa(-) mice exhibited perinatal lethality, and significantly higher levels of inosine in cellular RNA and deoxyinosine in nuclear DNA were detected in Itpa(-) embryos than in wild-type embryos. Therefore, we examined the effects of ITPA deficiency on mouse embryo...

Drug-induced immune thrombocytopenia (DITP) is caused by drug-dependent antibodies (DDAbs) that are nonreactive in themselves but bind tightly to specific platelet membrane glycoproteins (GP) when soluble drug is present at pharmacologic concentrations. This reaction takes place without covalent linkage of drug to the target, indicating that drug does not function as a classical hapten to promo...

• Quinine binds to quininedependent antibodies first and causes CDR loop reconfiguration. • A hybrid paratope consisting of quinine and reconfigured CDR recognizes its target epitope. Drug-induced immune thrombocytopenia (DITP) is caused by antibodies that react with specific platelet-membrane glycoproteinswhen the provokingdrug is present.More than 100 drugs have been implicated as triggers fo...

Drug-induced immune thrombocytopenia (DITP), excluding heparin-induced thrombocytopenia, is relatively uncommon. It is characterized by drug-dependent antibodies that bind to the platelets and cause their destruction when the responsible drug is ingested or injected. Imipramine is a tricyclic antidepressant drug that is one of those used widely for primary enuresis nocturna, attention deficit h...