Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined...

many factors may influence the efficiency of somatic embryogenesis. this capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. the effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mm) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

Having produced the embryos of cactus Copiapoa tenuissima Ritt. forma monstruosa at the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100  μ M on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA ...

somatic embryogenesis is a new approache to plant mass in vitro propagation. this method influences by elements and their components in medium. in order to investigate the effects of different media and it,s role on somatic embryogenesis of carrot, a cbd experiment with four replications (including 12 treatments) was conducted. to do this, carrot petioles (with 1 cm dimension) in induction phas...

to explore the potential for in vitro rapid regeneration of fagonia indica burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on ms medium which supplemented with 1.0-5.0 µm 2,4-d. callus was initiated from cotyledon explant on ms medium supplemented in all concentrations of 2,4-d investigated. 2,4-d was more effective than iaa and naa for cal...

Node, internode, and leaf explants of Populus deltoides Bartr. ex Marsh. × Populus deltoides Bartr. ex Marsh. hybrid poplar clone (89 M 066) tissue culture regenerated plantlets were used for direct and indirect somatic embryogenesis. Direct somatic embryos were differentiated on node, internode and leaf explants on Murashige and Skoog basal medium (MS) supplemented with N 6 -Benzyladenine (BA)...

somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...