An efficient and bench-stable reagent was synthesized for direct and covalent introduction of tetrazines onto target protein or virus surfaces, which can be further modified based on tetrazine-ene ligation to achieve fluorescence labelling or PEGylation under mild conditions.

Investigations into the cellular processing of radiolabeled monoclonal antibodies (mAbs) for their further use in radioimmunodiagnosis and cancer therapy are needed in order to understand the fate of internalized and catabolized mAbs. The anti-colorectal cancer mAb, A33, was labelled with 76Br and 125I using the direct Chloramine-T method, or by labelling N-succinimidyl para-(tri-methylstannyl)...

Given a graph G with n vertices and an Abelian group A of order n, an A-distance antimagic labelling of G is a bijection from V (G) to A such that the vertices of G have pairwise distinct weights, where the weight of a vertex is the sum (under the operation of A) of the labels assigned to its neighbours. An A-distance magic labelling of G is a bijection from V (G) to A such that the weights of ...

The rhodamine derivatives tetramethyl-rhodamine-5/6-maleimide (TROMI) and tetramethyl-rhodamine-6-isothiocyanate (TRITC) were allowed to react with living Hydra vulgaris. The two fluorescent dyes stain the polyps to different degrees, apparently without impairing their viability and behaviour. Concerning nematocytes, TROMI preferentially couples to cytoskeletal elements only of mounted nematocy...

Two human subjects were given separate oral doses of sodium [2H6]isobutyrate and [methyl-2H3]thymine and the labelling patterns of urinary metabolites were determined. Ingestion of deuterated isobutyrate resulted in the excretion of 2H5-labelled S-3-hydroxyisobutyric acid, formed on the direct catabolic pathway, and of S- and R-[2H4]-3-hydroxyisobutyric acids, formed by the reduction of S- and ...

Protein palmitoylation is a critical post-translational modification important for membrane compartmentalization, trafficking and regulation of many key signalling proteins. Recent non-radioactive chemo-proteomic labelling methods have enabled a new focus on this emerging regulatory modification. Palmitoylated proteins can now be profiled in complex biological systems by MS for direct annotatio...

This paper deals with automatic structuring and sentence boundary labelling in natural language texts. We describe the implemented structure tagging algorithm and heuristic rules that are used for automatic or semiautomatic labelling. Inside the detected sentence the algorithm performs a decomposition to clauses and then marks the parts of text which do not form a sentence, i.e. headings, signa...

In this work we introduce a novel solution to the semantic image labelling problem, i.e. the task of assigning semantic object class labels to individual pixels in a test image. Conventional methods are typically relying on random fields for modelling interactions between neighboring pixels and obtaining smooth labelling results using unary and pairwise cost functions. Instead, we consider the ...

We address the problem of transferring semantic annotations to new languages using parallel corpora. Previous work has transferred these annotations on a token-to-token basis, an approach that is sensitive to alignment errors and translation shifts. We present a global approach to transfer that aggregates information across the whole parallel corpus and leads to more robust labellers. We build ...

Ptd(4,5)P(2) is thought to promote and organize a wide range of cellular functions, including vesicular membrane traffic and cytoskeletal dynamics, by recruiting functional protein complexes to restricted locations in cellular membranes. However, little is known about the distribution of PtdIns(4,5)P(2) in the cell at high resolution. We have used the pleckstrin homology (PH) domain of phosphol...