Objectives: To explore the expression of maternal serum IgG anti-A (B) and neonatal direct anti globulin, free antibody and Red Blood Cell (RBC) antibody on the occurrence and development of ABO Haemolytic Disease of the New-born (ABO-HDN) and provide a valuable reference for the early diagnosis of the disease. Methods: we selected 546 cases of jaundice and hyperbilirubinemia neonates in our ho...

The serological and physicochemical properties of the following three forms of human anti-gamma-globulin factors were compared: (a) rheumatoid factors; (b) Milgrom type anti-gamma-globulin factors; and (c) factors directed against an antigen in human gammaG-globulin that is hidden in the intact molecule and revealed by enzymatic digestion at low pH. The property common to these factors is abili...

A study of the incidence of Rh-sensitization showed a 6.5% incidence of anti-D appearing for the first time during the last trimester of pregnancy. In 24 of the 29 subjects these antibodies were weak, and were found only when a sensitive technique using enzyme-treated Rh-deletion test cells was employed. The remaining five, however, had high-titre antibodies, which were associated with a positi...

A 65-year-old male with advanced colorectal cancer treated by sigmoidectomy and FOLFOX 6 regimen was admitted to the ICU after five cycles because of anuric acute renal failure (ARF) and thrombocytopenia (Table 1). Chemotherapy consisted in a combination of OXP, 5-fluorouracil (5-FU) and folinic acid. He had received a total dosage of 470 mg of OXP when shortly after the last infusion he compla...

Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]methionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit gamma-globulin antisera. A 15S mRNA has been found to direct synthesis of a 45 000 m...

Resting murine B lymphocytes can present rabbit anti-Ig to T cell lines specific for normal rabbit globulin. The T cell-B cell interaction is major histocompatibility complex (MHC)-restricted, and leads to activation, proliferation, and differentiation of the resting B cell into an antibody-secreting cell. Efficient antigen presentation and B cell activation depends upon binding of rabbit globu...

Equine immunoglobulin was detected along the glomerular basement membrane of three human homograft recipients who had been treated with equine anti-lymphocyte globulin. Anti-lymphocyte globulins, given these patients, were obtained by immunization of horses with lymphocytes from human spleens and/or lymph nodes and contained glomerular basement membrane-reactive antibodies. Quantitative paired-...

BACKGROUND A combination of horse anti-thymocyte globulin and cyclosporine produces responses in 60-70% of patients with severe aplastic anemia. We performed a phase II study of rabbit anti-thymocyte globulin and cyclosporine as first-line therapy for severe aplastic anemia. DESIGN AND METHODS Twenty patients with severe aplastic anemia treated with rabbit anti-thymocyte globulin were compare...