The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure durin...

Abstract The genus Schinus (Anacardiaceae) comprises 30 species found in Peru, Bolivia, Chile, Paraguay, Uruguay, Argentina and Brazil. terebinthifolia (Brazilian pepper tree) is socioeconomically important the Brazilian state of Espírito Santo. Dried fruit used as a pepper-like spice natural populations are exploited. Studies on meiotic behavior pollen stainability from an ecological evolution...

OBJECTIVE To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. DESIGN Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. SETTING Academic andrology laboratory. PATIENT(S) Men presenting for semen analysis. INTERVENTION(S) Washed sperm fractions of 32 ...

Pronuclei formation is routinely assessed 16-20 h after oocyte insemination in in-vitro fertilization (IVF). Occasionally, the pronuclei disappear before this time, rendering them as 'undocumented'. Since the number of pronuclei detected is used to distinguish normal from abnormal embryos in the context of ploidy, the diploidy of undocumented embryos is questionable, and therefore they are rout...

Philadelphia chromosome is observed in 25-50% of adult Acute Lymphoblastic Leukemia (ALL) cases and in about 5% of childhood ALL cases. A hyper diploid karyotype, defined by the presence of more than 46 chromosomes, is detected in 2-9% of adult ALL patients and in 29% of pediatric patients [1]. Translocation t (9; 22) or Philadelphia chromosome is a common structural aberration found in patient...

In a previous theoretical study we investigated whether adaptive or non-adaptive processes are more important in the evolution of senescence. We built a model that combined both processes and found that mutation accumulation is important only at those ages where mortality has a negligible impact on fitness. This model, however, was limited to haploid organisms. Here we extend our model by intro...

Normal cells, both in vivo and in vitro, become quiescent after serial cell proliferation. During this process, cells can develop immortality with genomic instability, although the mechanisms by which this is regulated are unclear. Here, we show that a growth-arrested cellular status is produced by the down-regulation of histone H2AX in normal cells. Normal mouse embryonic fibroblast cells pres...