Rat liver microsomal dihydroxyacetone phosphate acyltransferase, in contrast to the glycerophosphate acyltransferase, was found to be active at low pH (5.5), stable towards heat (55 degrees C, 15 min) and trypsin (in the absence of detergents) and was not inhibited by high concentrations of N-ethyl maleimide. Dihydroxyacetone phosphate acyltransferase is only slightly and non-competitively inhi...

1. Fat-free homogenates from the epididymal fat-pads of rats were used to measure the rate of palmitate esterification with different substrates. The effectiveness of the acyl acceptors decreased in the order glycerol phosphate, dihydroxyacetone phosphate, 2-octadecenyl-glycerol and 2-hexadecylglycerol. 2. Glycerol phosphate and dihydroxyacetone phosphate inhibited their rates of esterification...

Glycerol and dihydroxyacetone are metabolized by rabbit kidney-cortex tubules, isolated by collagenase treatment. Half-maximal concentrations of both substrates were determined with regard to uptake rates and product formations. Maximal uptake rates were 643 and 329 mumol/h per g of protein for dihydroxyacetone and glycerol respectively. Glucose and lactate were found as major metabolic product...

Dihydroxyacetone phosphate was prepared in 2OGmmol scale from dihydroxyacetone by two procedures: reaction with phosphorus oxytrichloride and glycerol kinase catalyzed phosphorylation using ATP with in situ regeneration of ATP by phosphoenolp5rruvate or acetyl phosphate. Dihydroxyacetone phosphate was converted to fructose 6-phosphate in 80% yield by exposure to a mixture of co-immobilized trio...

In resting Acetobacter suboxydans cells, glycerol can be oxidized beyond the dihydroxyacetone stage, and sorbitol, beyond the sorbose stage (King and Cheldelin, 1952a). 2 ,4-Dinitrophenol inhibits the further oxidation of dihydroxyacetone or sorbose but has no effect upon the first step oxidations (King and Cheldelin, 1952b). Since these keto derivatives can be metabolized further by resting ce...

1. The changes in the metabolite content in the isolated perfused rat liver and in the perfusion medium were measured after loading the liver with glycerol or dihydroxyacetone. 2. Glycerol was rapidly taken up by livers from fed and starved rats; glucose, lactate and pyruvate were discharged into the medium. The [lactate]/[pyruvate] ratio in the medium rose from 10 to 30 and in the tissue from ...

Phosphatidic acid is synthesized by a particulate preparation of of guinea pig liver from dihydroxyacetone phosphate, acyl CoAs and NADPH via acyl dihydroxyacetone phosphate. Glycerol-3-phosphate is also converted to phosphatidate in this system. The phosphatidate formed via acyl dihydroxyacetone phosphate has more saturated fatty acid in the lposition than in the Z-position, while the fatty ac...

In work previously reported from this laboratory it was found that CWlabeled glycerol, completely hydrolyzed from glycerides during digestion, was not utilized for the resynthesis of lymph glycerides (1). This suggests that glycerol per se may not be the precursor of glyceride glycerol. Since absorbed fatty acids appear in the lymph as triglycerides, it is necessary to account for the origin of...