Dihydrofolate reductase (DHFR) catalyzes folic acid reduction and recycles dihydrofolate generated during dTMP biosynthesis to tetrahydrofolate. DHFR is the main target of methotrexate, the most widely used agent for antifolate therapy. Nevertheless, the emergence of methotrexate-resistance has greatly impeded the curative potential of this drug. Therefore, drugs with improved efficacy are stil...

Dihydropteroate synthase (DHPS) gene mutations have raised concerns about emerging sulfonamide resistance in Pneumocystis jirovecii. DHPS and dihydrofolate reductase (DHFR) gene products were amplified in clinical specimens from South African patients. One of 53 DHPS genes sequenced contained the double mutation Thr55Ala Pro57Ser. DHFR gene mutations detected were Ala67Val and the new mutations...

Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-Acetylphenylalanine was incorporated into recombinant dihydrofolate reductase (DHFR) in Escherichia coli via a computationally designed mutant form of the phenylalanyl-tRNA synthetase of the host. DHFR outfitted with ketone functionality can be chemoselectively ligated with hydraz...

In Saudi Arabia, Origanum vulgare is widely disseminated. the present work, we used GC-MS analysis to identify components of essential oil. The disc diffusion assay was assess oil’s in vitro antibacterial efficacy against Gram-positive and Gram-negative Staphylococcus aureus Escherichia coli. antimicrobial many microbial proteins, including tyrosyl-tRNA synthetase (TyrRS), DNA gyrase, dihydrofo...

Dihydrofolate reductase (DHFR) enzyme is a crucial component of cell growth and proliferation in the human body, making it an important target for treating cancer diseases. This study aims to predict inhibitory activity (pXC50) dihydrofolate inhibitors terms quantitative structure-activity relationship (QSAR) model. Interpretation QSAR model vital understanding physicochemical processes assist ...

We have determined the nucleotide sequence of the dihydrofolate reductase-thymidylate synthetase (DHFR-TS) gene of the protozoan parasite Leishmania major (dihydrofolate reductase, EC 1.5.1.3 and thymidylate synthase, EC 2.1.1.45). The DHFR-TS protein is encoded by a single 1560-base-pair open reading frame within genomic DNA, in contrast to vertebrate DHFRs or mouse and phage T4 TSs, which con...

Dihydrofolate reductase (DHFR) plays an essential role in cell growth and proliferation. In the cellular sole de novo thymidylate biosynthesis cycle, DHFR and thymidylate synthase (TS) catalyze sequential reactions, leading to formation of thymidine monophosphate — a substrate indispensable for DNA synthesis. Taking into account that posttranslational modifications, such as phosphorylation, can...

The goal of this study was to genetically link natural materials derived from Tinospora crispa L with Berberine dihydrofolate reductase-thymidylate synthase (DHFR-TS). Method: ligand berberine (CID: 2353) obtained Pubchem, while the protein DHFR-TS (PDB ID 2bl9) Protein Data Bank. ligands and proteins interacted HEX 8.0.0.0 were visualized Discovery Studio. researchers discovered a positive int...

This study examined polymorphisms in the dihydrofolate reductase (DHFR) gene of Pneumocystis carinii isolates from 27 patients with P. carinii pneumonia (PCP) in Japan. Four substitution sites with two synonymous and two non-synonymous changes were found. Two synonymous substitutions at nucleotide positions 540 and 312 were identified in one and 13 patients, respectively. Two amino acid substit...

Cultured human KB cells which developed resistance to increasing concentrations of methotrexate showed increased levels of dihydrofolate reductase (DHFR) as demonstrated by an immunobridge staining technique. Peripheral blast cells from a leukemic patient were examined for DHFR content during several courses of methotrexate therapy. The number of cells which demonstrated high levels of DHFR inc...