Protease inhibitors, which can inhibit insect digestive enzymes, and carbohydrate binding lectins are potential control mechanisms against herbivorous insects. Larval midgut extracts fi-om Eldana saccharina Walker (Lepidoptera: Pyralidae) were assayed for protease activity in the presence of protease inhibitors in order to determine the dominant protease types (serine, aspartic, metalloor cyste...

Background: Determination of digestive enzymes activity would provide critical information in the design of appropriate diet. Sobaity, Sparidentex hasta is found in the Persian Gulf and  cultured in countries adjacent to it. OBJECTIVES: This study investigated proteolytic, amylase and lipase activities in the intestine of Sparidentex hasta captured from the  Persian Gulf. METHODS: 60 immature S...

trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. trypsin has been used widely in various biotechnological processes. in this study trypsin was purified using two precipitation cut, 35% and 75% as saturation percent of ammonium sulfate solutions, tca precipitation (2.5% w/v), ph precipitation and finally affinity chromatography. this m...

the present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticle on general performance and digestive proteases in zebra fish. four experimental diets were designed with a concentration of 5, 20 and 40 parts per billion (ppb) nanoparticles in the diet (named s5, s20, and s40, respectively) and control group without any nanoparticles (s0). there was no significa...

Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands and their apparent molecular weights were identified in both tissue extracts and luminal contents by gel zymography: (1) a 125 kDa protease in sali...

Cockles Cerastoderma edule were fed two different concentrations of two diets with different qualities which were achieved by mixing different proportions of ashed silt particles with cells of the microalgae Tetraselmis suecica. After 3 days (acute response) and 11 days (acclimated response) of exposure to the diets, we analysed the digestive activity of the digestive gland using cyto-histologi...

Enteropeptidase, a type II transmembrane serine protease, is localized to the brush border of the duodenal and jejunal mucosa. It is synthesized as a zymogen (proenteropeptidase) that requires activation by another protease, either trypsin or possibly duodenase. Active enteropeptidase then converts the pancreatic precursor, trypsinogen, to trypsin by cleavage of the specific trypsinogen activat...