Several studies have reported data on comparisons between two methods: the total collection of feces and the internal markers method. The aim of this study was to assess the apparent digestibility of two concentrates and to compare the apparent digestion coefficients using the total collection of feces and acid-insoluble ash (AIA) as the internal marker method. In 2009, six adult geldings aged ...

Short-term (h) and long-term (days) individual variability and the effects of momentary change in feeding intensity on digestion time were studied in the scyphomedusa Aurelia aurita as a basis for developing a method to experimentally measure the digestion rate with a high precision. Ten individual medusae showed only small, non-significant differences in average digestion time (range, 2.1-2.5 ...

Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in ...

Elevating the reaction temperature is an effective method to accelerate protein enzymatic digestion because it can promote protein denaturation and enzyme activities. In this study, we demonstrated a new photothermal heating method to assist protein tryptic digestion on glass slides. A glass slide coated with layer-by-layer gold nanoparticles (Glass@AuNPs), combined with the use of a near infra...

Jean Dumas (Fig. 1) published his combustion method in Paris back in 1848. This makes the method considerably older than Kjeldahl digestion. While researching the composition of natural oils, Dumas tried combusting his samples, initially to analyse the carbon and hydrogen contained therein. Combustion therefore makes it possible to analyse multiple parameters, while Kjeldahl digestion is only s...