Optimal reaction conditions to sassay human lactate dehydrogenase (lactate-to-pyruvate) were established for isoenzymes 1 and 5 at 25, 30, and 37 degrees C in diethanolamine and 2-amino-2-methyl-1,3-propanediol. Different substrate concentrations are required at each temperature. The conditions permit measurement of lactate dehydrogenase 1 and 5 with the lowest substrate concentrations that all...

The antimicrobial effects of diethanolamine, dimethylamino-methyl-propanol, and butylethanolamine are greatly enhanced at high pH. Their antimicrobial activities are closely correlated with their uncharged forms, indicating that diffusion through cell membrane(s) is rate limiting for the antimicrobial action. Since these compounds are selectively toxic at high pH, they are appropriate antimicro...

Influence of Gallogen and Diethanolamine Upon Induced Hypercholesterolemia in the Rat Print ISSN: 0009-7330. Online ISSN: 1524-4571 Copyright © 1958 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Circulation Research doi: 10.1161/01.RES.6.3.271 1958;6:271-274 Circ Res. http://circres.ahajournals.org/...

New-type three-component cationic hybrid liposomes (HLs) composed of dimyristoylphosphatidylcholine (DMPC), polyoxyethylene(21)dodecyl ether (C(12)(EO)(21)) and O,O'-ditetradecanoyl-N-(α-trimethylammonioacetyl) diethanolamine chloride (2C(14)ECl) were produced. Cationic HLs were smaller and more stable than pure DMPC liposomes. It is noteworthy that cationic HLs could remarkably inhibit the gro...

Catalysis and inhibitor binding by the GH43 beta-xylosidase are governed by the protonation states of catalytic base (D14, pK(a) 5.0) and catalytic acid (E186, pK(a) 7.2) which reside in subsite -1 of the two-subsite active site. Cationic aminoalcohols are shown to bind exclusively to subsite -1 of the catalytically-inactive, dianionic enzyme (D14(-)E186(-)). Enzyme (E) and aminoalcohols (A) fo...

The purpose of the present experiments was to test the hypothesis that diethanolamine (DEA), an alkanolamine shown to be hepatocarcinogenic in mice, induces hepatic choline deficiency and to determine whether altered choline homeostasis was causally related to the carcinogenic outcome. To examine this hypothesis, the biochemical and histopathological changes in male B6C3F1 mice made choline def...

The release of gentamicin sulphate, sodium fusidate and diethanolamine fusidate from Palacos and CMW cements was studied using elution and serial plate transfer tests. Further tests were made to assay the drug remaining in the cement after antibacterial activity could no longer be detected by the above methods, to detect the sustained slow release of the residual drug, and to ascertain the mech...

Diethanolamine-Induced Alteration of Hepatic Mitochondrial Function and Structure. BARBEE, S. J., AND HARTUNG, R. (1979). Toxicol. Appl. Pharmacol. 47,431-440. Diethanolamine (DEA) has been shown to interfere with phospholipid metabolism. It was hypothesized that DEA may alter membrane-bound biochemical processes which are dependent upon phospholipids. DEA was investigated for its effect on hep...

Hesperaloe funifera was characterized in terms of its contents of holocellulose, α-cellulose, and lignin (74.1, 52.3, and 7.9%, respectively). Also, an experimental factor design was used to examine the influence of operational variables in the diethanolamine pulping of this material (viz. diethanolamine concentration (50-80%), cooking temperature (155-185 oC), and time (30-90 minutes)) on the ...