This work shows that single sequencing reactions analyzed on an automated DNA sequencer can be an efficient way of screening PCR products for known mutations. We have analyzed a mutation in exon 10 of the human aromatase gene and show that an unambiguous genotype could be elucidated in more than 90% of the analyzed samples. Compared to analysis by full sequencing, 4 times more samples can be an...

A rapid and simplified fluorescence in situ hybridization (FISH) technique for the detection of human chromosome-specific centromeric probes is described. Using chromosomes 1-, 4-, 11and Y-specific fluorescence-labeled probes, the modified, or quick-FISH technique, was compared to two conventional FISH methods. The modified FISH technique detected human chromosomes without sacrificing sensitivi...

introduction: rapid diagnosis of traumatic intrathoracic injuries leads to improvement in patient management. this study was designed to evaluate the diagnostic value of chest radiography (cxr) in comparison to chest computed tomography (ct) scan in diagnosis of traumatic intrathoracic injuries. methods: participants of this prospective diagnostic accuracy study included multiple trauma patient...

Turbochargers are most widely used in automotive, marine and locomotive applications with diesel engines. To increase the engine performance nowadays, in aerospace applications also turbochargers are used. Mostly the turbocharger rotors are commonly supported over the fluid film bearings. With the operation, lubricant properties continuously alter leading to different load bearing capacities. T...