نتایج جستجو برای: diafiltration yield
تعداد نتایج: 194301 فیلتر نتایج به سال:
An improved method for the isolation of the biosurfactant glycolipids from Rhodococcus sp. strain H13A by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does not contribute any biosurfactant char...
BACKGROUND Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia and now is being recognized as an important neurotropic virus. Effective medications and prophylactic vaccine against EV71 infection are urgently needed. Based on the success of inactivated poliovirus vaccine, a prototype chemically inactivated EV71 vaccine candidate has been developed ...
Nowadays the waste from protein fibres represents an important renewable source for a new generation of biomaterials and promising competitors for carbohydrate based biomaterials. Regenerated keratin biomaterials are biodegradable in vivo and in vitro, biocompatible, and support cell attachment and proliferation; however, their major drawback has been their weak mechanical properties such as du...
Application of membrane technologies in biorefinery processes has been studied for some time. The heterogenous nature steams, however, results unideal performance systems and considerable fouling membranes, which is decreasing the efficiency separation. As a part BioSPRINT project, this study focuses on application separating monomeric sugars from hemicelluloses fraction lignocellulosic biomass...
با پیشرفت تکنولوژی و کاهش ابعاد ترانزیستور، تلرانس پارامترهای ساخت مدارات الکترونیک افزایش یافته و باعث کاهش ضریب yield می شود. در طراحی مدارات الکترونیک، طراح عموما مقادیر نامی را برای دست یابی به عملکرد مطلوب استخراج می کند، در حالی که، در عمل یک ناحیه تلرانس، به دلیل فرآیند های تصادفی حین فرآیند های ساخت، وجود دارد که بایستی در طراحی لحاظ گردد. الگوریتم های بهینه سازی طراحی تلرانس، ناحیه تلر...
Solid-state fermentation has been widely used for enzyme production. However, secreted enzymes often bind to the solid substrate preventing their detection and recovery. A series of screening studies was performed to examine the role of extraction buffer composition including NaCl, ethylene glycol, sodium acetate buffer, and Tween 80, on xylanase and cellulase recovery from switchgrass. Our res...
The purification of virus particles and viral vectors for vaccine and gene therapy applications is gaining increasing importance in order to deliver a fast, efficient, and reliable production process. Ultrafiltration (UF) is a widely employed unit operation in bioprocessing and its use is present in several steps of the downstream purification train of biopharmaceuticals. However, to date few s...
Monoclonal antibodies (mAbs) are one of the fastest growing drug molecules targeting the treatment of diseases ranging from arthritis, immune disorders, and infectious diseases to cancer. Due to its unique application principle, antibodies are commonly produced in large quantities. Plants, such as Nicotiana benthamiana, offer a unique production platform for bio-therapeutics due to their abilit...
Purpose. The study reports evaluation of different purification processes for removing surplus surfactant and formulating stable nanoparticle dispersions. Methods. Nanoparticle formulations prepared from poly(D,L-lactide-co-glycolide) and polyvinyl alcohol (PVA) were purified by a diafiltration centrifugal device (DCD), using 300K and 100K molecular weight cut-off (MWCO) membranes and a tangent...
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