In a previous study, an in vitro cell-free assay of translation inhibition by H-2 toxin, HT-2 and deoxynivalenol using commercially available kit was developed. They showed significant the system. For further evaluation, I examined three additional modified compounds, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol-3-glucoside, this study. 15-acetyldeoxynivalenol inhibited as we...

Distiller's dried grains with solubles (DDGS) is a major coproduct of the fuel-ethanol industry and is becoming a popular low-cost ingredient for animal feed. Uncertainties regarding the risk factors in DDGS, such as level of mycotoxins, could limit its application in the animal feed industry. To provide a scientifically sound assessment of the prevalence and levels of mycotoxins in U.S. DDGS, ...

deoxynivalenol contamination was determined in corn produced in golestan and ardabil (moqan) provinces, iran, in 2004-2005. samples were collected from different stages of production, including before harvest, at harvest, post harvest as well as after drying. ground sub samples were extracted with water; each extracted sample was cleaned up through an immunoaffinity column. deoxynivalenol was e...

This study was undertaken in order to investigate the effect of deoxynivalenol on the oxidative status and antioxidant defense of HepG2 cells. The HepG2 cells were exposed to 2.5 μM deoxynivalenol for 6, 12 and 24 hours. Higher (p<0.01) activities of the antioxidant enzymes such as superoxide dismutase and catalase were observed after 12 and 24 hours of mycotoxin exposure, while, for the same t...

A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the Fusarium mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3-gluc...

Here we report the draft genome of Devosia sp. strain 17-2-E-8, isolated from Ontario agricultural soil (Canada) with promising deoxynivalenol biotransformation capabilities. In addition, we report the draft genome of Devosia riboflavina strain IFO13584, used as a control strain in our studies aimed at highlighting unique gene clusters involved in deoxynivalenol epimerization.

We report the identification of deoxynivalenol-3-sulfate and deoxynivalenol-15-sulfate as two novel metabolites of the trichothecene mycotoxin deoxynivalenol in wheat. Wheat ears which were either artificially infected with Fusarium graminearum or directly treated with the major Fusarium toxin deoxynivalenol (DON) were sampled 96 h after treatment. Reference standards, which have been chemicall...

The presence of masked or hidden forms of Fusarium mycotoxins (deoxynivalenol, DON, zearalenone, ZEN and fumonisins B1, B2 and B3) were studied in wheat and maize derived products. Significant amounts of these forms were found both in raw and in processed food commodities. Deoxynivalenol-3-glucoside was found in wheat products up to 30% of DON concentration. Bound forms of fumonisins often acco...

abstractthe occurrence of deoxynivalenol (don) in retail foods in tehran (iran) was determined using high-performance liquid chromatography technique and immunoaffinity column as the clean-up step. a method was validated for analysis of don in rice, bread, puffed corn snack and wheat flour. the average recoveries and precision (rsd) for don in different foods ranged 84.2-93.1% and 2.9-12.0%, re...

The contamination of consumer food and animal feed with toxigenic fungi has resulted in economic losses worldwide in animal industries. Mycotoxins are highly biologically reactive secondary metabolites and can inhibit protein synthesis and cell multiplication. Considering the cytotoxicity of mycotoxins, this experiment was performed to determine the in vitro influence of ochratoxin A, deoxyniva...