BACKGROUND Different methods have been used in order to isolate dental pulp stem cells. The aim of this study was to study the effect of different types of pulp treatment during isolation, under 3% O2 conditions, in the time needed and the efficacy for obtaining dental pulp stem cells. MATERIAL AND METHODS One hundred and twenty dental pulps were used to isolate dental pulp stem cells treatin...

introduction: dental pulp-derived mesenchymal stem cells (mscs) have emerged as a promising tool for use in regenerative medicine. the in vitro growth kinetic and culture requirement of the cells derived from human dental pulp, which is the subject of this present study, is poorly described. materials and methods: stem cells were derived from human third molar and then characterized. the in vit...

the purpose of this review is to gain an overview of the applications of the dental pulp stem cells (dpscs) in the treatment of various medical diseases. stem cells have the capacity to differentiate and regenerate into various tissues. dpscs are the adult   stem cells that reside in the cell rich zone of the dental pulp. these are the multipotent cells that can be explained by their embryonic ...

introduction based on previous researches, dental pulp stem cells (dpscs) are easily accessible with limited morbidity after collection. their embryonic origin, from neural crests, explains their multipotency. dpscs are primarily derived from the pulp tissues of the teeth.objective: this study was undertaken to isolate, culture, and characterize two different third molar and first premolar huma...

objectives: this study aimed to compare the in vitro cytotoxic activity of propolis, a bioactive material made by the honeybee, and calcium hydroxide (ch) and their effect on formation of mineralized nodules by human dental pulp stem cells (hdpscs). methods: in this in vitro study, hdpscs were obtained from the cellular and molecular oral biology laboratory of school of dentistry, shahid behesh...

considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. in enzymatic digestion method extracted teeth as denta...

In the past few years there have been significant advances in the identification of putative stem cells also referred to as "mesenchymal stem cells" (MSC) in dental tissues including the dental pulp. It is thought that MSC in dental pulp share certain similarities with MSC isolated from other tissues. However, cells in dental pulp are still poorly characterized. This study focused on the charac...

It is a challenge for dentists to save dental pulp in patients with pulp disease without resorting to root canal therapy. Formation of tertiary dentin to maintain pulp vitality is a key odontoblast response to dental pulp injury. Vascular endothelial growth factor (VEGF) is the most potent angiogenic and vasculogenic factor involved in tertiary dentin formation. It was hypothesized that VEGF ma...

objective: pulp and periodontal tissues are well-known sources of mesenchymal stem cells (mscs) that provide a promising place in tissue engineering and regenerative medicine. the molecular mechanisms underlying commitment and differentiation of dental stem cells that originate from different dental tissues are not fully understood. in this study, we have compared the expression levels of pluri...

Dental Caries affects approximately 90% of the world's population. At present, the clinical treatment for dental caries is root canal therapy. This treatment results in loss of tooth sensitivity and vitality. Tissue engineering can potentially solve this problem by enabling regeneration of a functional pulp tissue. Dental pulp stem cells (DPSCs) have been shown to be an excellent source for pul...