objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

Background: Dendritic cells (DCs) are professional antigen presenting cells that have an important role in the initiation of immune response. The use of maturation factors in dendritic cell differentiation provides a promising approach in immunotherapy. Objective: In this study, we compared tumor necrosis factor-α, polyribocytidylic acid, lipopolysacharide and CpG oligonucleotides in inducing d...

variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. in this research, we studied the capacity of dendritic cells to expand antigen-specific t regulatory cells.we also investigated the role of tgf-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions. dendritic cells were generated f...

objective(s) recent studies on human indicate that the introduction of therapeutic use of tolerogenic dendritic cell (dc) for chronic inflammatory conditions is imminent. for the purpose of defining cgrp potency in tolerogenic dc production, we investigated the phenotype and il-'2 production of dcs generated from the monocytes of rheumatoid arthritis (ra) patients in the presence of the ca...

hla-g is normally expressed on human trophoblast cells. it is a non-classical mhc molecule class i b. the role of hla-g in diabetic type 1 is not known. we investigated the role of ifn-β in induction hla-g expression on the monocyte derived dendritic cells (dc) in diabetes type 1. treatment of dendritic cell with ifn-β in vitro from diabetic patients (n=20) and normal subjects (n=20) resulted t...

during antigen capture and processing, mature dendritic cells (dc) express large amounts of peptide-mhc complexes and accessory molecules on their surface. we investigated the role of ifn-β in induction hla-g expression on the monocyte derived dc and cytokine profile in diabetes type 1. we accomplished secretary pattern and total cytokine production of the th1 cytokine (il-2, γifn) and th2 cyto...

Introduction: Despite considerable efforts to control AIDS pandemic, it is still one of the significant infectious concerns worldwide. The advance in medical research has led to the development of highly active antiretroviral therapy with a considerable effect to suppress the disease. However, an effective vaccine capable of eradication the HIV pandemic is not available yet. Failure to develop ...