Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We used activation tagging to isolate a dominant mutation affecting release from dormancy and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and retransformation experiments that EB...

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

The growth of the top-leader in Abies nordmanniana was measured over a 6-year period, and showed consistent pattern, with an initial phase reaching maximum rate that varied between 12.0 22.5 mm per day, which could be correlated temperature July previous year. daily always peaked last week June. In late-bud-breaking plants, became short because low this first growth. second phase, slowly declin...

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

The present study investigated the expressional regulation of PpDAM5 and PpDAM6, two of the six peach (Prunus persica) dormancy-associated MADS-box genes, in relation to lateral bud endodormancy. PpDAM5 and PpDAM6 were originally identified as homologues of Arabidopsis SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 identified in the EVERGROWING locus of peach. Furthermore, PpDAM5 and PpDAM6 have recent...

a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was al...