Alkaline protease producing microorganism Vibrio metschnikovii NG155, isolated from the soil samples of leather industry, having potential application in dehairing of animal skin was used in this study. To enhance the production of protease by this strain, various physical and nutritional factors were optimized using statistical methods like Plackett Burman and Response Surface Methodology. In ...

An antigenic glycosylated cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. It exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. This enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by t...

Two novel extracellular keratinases were produced by Actinomadura keratinilytica strain Cpt20. Both enzymes purified to homogeneity using heat-treatment (60 °C for 30 min) and ammonium sulfate salt fractionation (40 %–70 %), followed anion-exchange chromatography with fast protein liquid (FPLC) system. The keratinases, designated as KERA-71 KERB-19, are monomeric named according their molecular...

Conventional dehairing methods in the traditional leather-making processes, consume large amounts of toxic chemicals and produce a sludge/effluent, posing disposal challenges consequently environmental pollution. The by-products leather processing such as hair fat, contain chemicals. In this study, crude alkaline protease from <i>bacillus cereus strain 1-p, </i>was used to dehair sh...