BACKGROUND The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material is necessary. METHODS Pairs of bilateral bone marrow biopsies sampled from 53 patients were de...

INTRODUCTION Enamel demineralization is a problem in orthodontics. Fluoride is partially effective in addressing this problem, but additional treatment options are needed. The objective of this prospective randomized controlled trial was to determine the effectiveness of a new product, MI Paste Plus (GC America, Alsip, Ill), in the prevention or reduction of white spot lesions in orthodontic pa...

A prospective clinical trial was carried out to compare argon laser-curing of a traditional light-activated composite resin with conventional visible light-curing in terms of bond failure rate and incidence of enamel decalcification. Forty-five patients with a total of 742 metal brackets bonded to the upper and/or lower teeth in a modified split-mouth design participated in the study. The adhes...

Aim. In routine histopathology, decalcification is an essential step for mineralized tissues. The purpose of this study is to evaluate the effects of different decalcification solutions on the morphological and antigenicity preservation in Sprague Dawley (SD) rat femurs. Materials and Methods. Four different decalcification solutions were employed to remove the mineral substances from rat femur...

Vascular calcification is an important risk factor for cardiovascular disease, and is closely associated with all-cause mortality. Recently, it has been revealed that vascular calcification is not a passive precipitation of circulating minerals, but is a process actively regulated through machinery similar to bone formation. During the bone remodeling, osteoclasts execute the bone resorption by...

Hydroxyapatite (HA) has polarization capability and is able to accumulate electrical storage in bone. Experiments were conducted to measure the polarization capability of rabbit femurs. After preparing and polarizing bone samples using 2% KOH treatment (denoted 2% koh), 2% KOH and baking (2% koh+bake) and decalcification (decalcification) as well as untreated bone (untreated), stored charges we...

Cement paste leaching and concomitant decalcification is a common cause of concrete structure deterioration. Such alterations entail microstructural changes that condition durability. This study analysed those in cement pastes exposed to accelerated decalcification, layer-by-layer, from the surface contact with agent, 6 M NH4NO3, unaltered area. The were BSEM, MIP BET whilst mineralogical nanos...

The dental erosion or decalcification of enamel is a significant clinical problem. Apple acidic beverages are thought to increase the potential for dental erosion. The purpose of this in vitro study was to investigate the effect of clove essential oil (CEO) and its active principles on tooth decalcification of apple juices. On GC-MS analysis, CEO showed a high content of eugenol (58.29%) and eu...

This prospective, in vivo study compared bond failure and enamel decalcification with a cyanoacrylate bracket bonding system (SmartBond, Gestenco International, Gothenburg, Sweden) and a traditional light-cured composite system (Light Bond, Reliance Orthodontic Products, Itasca, Ill). A total of 327 teeth were evaluated after a period of 12 to 14 months; 163 experimental teeth were bonded with ...

BACKGROUND Aortic valve decalcification by ultrasound was given up. We evaluated a new ultrasound microhandpiece (Dissectron Penstyle) to rehabilitate this alternative treatment. METHODS We used under magnifying lenses the ultrasound microhandpiece to decalcify 30 explanted aortic valves. In the cases with embedded calcifications the thin top of the probe could be introduced into the thicknes...