Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red the most widely used substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular improved sensitivity and selectivity HRP thus desired. Herein, three dihydroflu...

Localization and staining features of the oxidant-sensitive fluorescent probe 2'7'-dichlorofluorescin (DCFH) were evaluated in isolated cardiac muscle cells. Cardiomyocytes rapidly accumulated the probe and retained steady levels of DCFH and its highly fluorescent oxidized product dichlorofluorescein (DCF) in probe-free medium for 1.5 h. DCF was associated with mitochondria and was released by ...

One of the objectives of studying endothelial cells in vitro is to evaluate neutrophil-endothelial cell interactions including potential consequences of oxidant-mediated damage to the endothelial cell. Current understanding of endothelial cell oxidative function is derived primarily from the measurement of extracellular products. We utilized 2 dyes, 2',7'-dichlorofluorescein diacetate (DCFH-DA)...

Introduction: Anti-inflammatory drugs are being utilized to treat cancer because of its inflammatory microenvironment. Objective: The objective this study is investigate the antioxidant potential indomethacin and genotoxicity, since free or loaded in polymeric nanocapsules using MCF-7 (human breast cancer) cells as an vitro model. Method: Development indomethacin-loaded polyepsiloncaprolactone ...

Opioids generate free radicals that mediate protection in isolated cultured cardiomyocytes. We hypothesize that the nature of these radicals is nitric oxide, and that nitric oxide activates the protein kinase C (PKC) delta isoform. Through this signal transduction pathway, opiates protect cardiomyocytes during hypoxia and reoxygenation. Cell viability was quantified in chick embryonic ventricul...

Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...

The present study addressed the effects of Mn on oxidative stress in a catecholaminergic CATH.a cell line. Oxidative stress was measured with the fluorescent dye, 2',7'-dichlorofluoroscein (DCFH). In the diacetate form, the dye is taken up by the cells and cleaved by esterases, effectively trapping it within the intracellular space. DCFH is subsequently oxidized treatment in the presence of rea...

The studied hypothesis is that the herbicide glyphosate (GLY) can affect oxidative balance in hydrophobic intracellular medium non-target Chlorella vulgaris cells. Analytical GLY and RoundUp (RUP) supplementation, affected growth profile. A significant 42% decrease cellular biomass stationary (St) phase was observed cultures supplemented with either 5 µM of or RUP, as compared to control cultur...

widely utilized to examine ROS levels. However, measurements based on redox-sensitive dyes such as DCFH can be problematic because they depend on dye uptake and lack any specificity towards a particular type of ROS. The advent of protein-based redox sensors like redox-sensitive GFP (roGFP) have improved specificity to particular ROS and can be targeted to different compartments within the cells...