نتایج جستجو برای: dargazi and gh1
تعداد نتایج: 16827227 فیلتر نتایج به سال:
Harnessing the biotechnological potential of the large number of proteins available in sequence databases requires scalable methods for functional characterization. Here we propose a workflow to address this challenge by combining phylogenomic guided DNA synthesis with high-throughput mass spectrometry and apply it to the systematic characterization of GH1 β-glucosidases, a family of enzymes ne...
To study the response of Iranian melon cultivars to salinity stress a factorial experiment based on RCBD with 3 replications was conducted. Four salinity treatments (0, 6.6, 8 and 12 dS/m NaCl( and 16 muskmelon (Cucumis melo L.) cultivars namely ‘Dargazi’, ‘Khatooni’, Abbas shori’, ‘Chah paliz’, ‘Zard Ivanaki’, ‘Gorgab’, ‘Gongi’, Saderati’, ‘Dastgerd’, ‘Sabz Isfahan’, ‘Jalali’, Ghasri Mashha...
We present an approach to evaluate the support for candidate genes as quantitative trait loci (QTLs) within the context of genome-wide map-based cloning strategies. To establish candidacy, a bacterial artificial chromosome (BAC) clone containing a putative candidate gene is physically assigned to an anchored linkage map to localise the gene relative to an identified QTL effect. Microsatellite l...
β-Glucosidases are enzymes that hydrolyze β-glycosidic bonds to release non-reducing terminal glucosyl residues from glycosides and oligosaccharides, and thus have significant application potential in industries. However, most β-glucosidases are feedback inhibited by the glucose product, which restricts their application. Remarkably, some β-glucosidases of the glycoside hydrolase (GH) 1 family ...
The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing. The secondary structure elements of GH1, three helices (S5-A16, S24-A34, N42-...
A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution the mutants F401S and N282T of Thermus thermophilus β-glycosidase (Ttβ-gly, glycoside hydrolase family 1 (GH1)), which display a h...
OBJECTIVE GH1 gene presents a complex map of single nucleotide polymorphisms (SNPs) in the entire promoter, coding and noncoding regions. The aim of the study was to establish the complete map of GH1 gene SNPs in our control normal population and to analyse its association with adult height. DESIGN, SUBJECTS AND MEASUREMENTS A systematic GH1 gene analysis was designed in a control population ...
The oligomeric state in solution of four plant annexins, namely Anx23(Ca38), Anx24(Ca32), Anx(Gh1), and Anx(Gh2), was characterized by sedimentation equilibrium analysis and gel filtration. All proteins were expressed and purified as amino-terminal His(n) fusions. Sequencing of the Anx(Gh1) construct revealed distinct differences with the published sequence. Sedimentation equilibrium analysis o...
Growth hormone (GH) was elevated in young growing, intact female Wistar-Furth rats bearing growth hormone (GH1) or growth hormone and prolactin (GH3) secreting tumors. Animals were injected with GH1 or GH3 cells at 1 wk of age. Total feed intake was measured for the 8-wk period from weaning until killed at 11 wk of age. Animals were fed a commercial chow diet throughout the trial. Body composit...
Depressive-like behaviors in animals are usually assessed by standardized behavioral tests such as the forced swimming test (FST). However, individual variation in test performance may obscure group differences and thereby hinder the discovery of genes responsible for depression. Few reports have shown the influence of individual variability in identifying the genes associated with depressive-l...
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