Loose, fibrillar, spherical structures have been observed during recent years in interphase nuclei of both animal and plant cells. These nuclear formations have been referred to as karyosomes, fibrillar bodies, micropuffs and centromeres. In order to gain further information on the nature of these structures, a cytochemical and radioautographic investigation was undertaken using plant meristema...

Palade, G.E. 1951. Intracellular localization of acid phosphatase: a comparative study of biochemical and histochemical methods. demonstration of the sites of activity of dehydrogenase systems with the electron microscope. A cytochemical study on the pancreas of the guinea pig: II. Functional variations in the enzymatic activity of microsomes. A cytochemical study on the pancreas of the guinea ...

Frontal organs and epiphyses of the pineal system from the adult frog, Rana pipiens, were fixed in s-collidine-buffered osmium tetroxide, embedded in Epon 812, and examined by electron microscopy. Epiphyseal material was also fixed in a variety of ways and subjected to a series of cytochemical tests for light microscopy. An ultrastructure resembling that of lateral eye retina is confirmed in th...

Terminal deoxynucleotidyl transferase activity, immunologic membrane markers, and cytochemical reactivity were examined in blasts of 80 adults with acute leukemia. The study was interinstitutional and prospective so that some assessment could be made of the usefulness and practicality of these tests in the classification of adult acute leukemias. Initial classification as to the type of acute l...

Examination of human gastric biopsies by an indirect immunofluorescence technique using antihuman gastrin confirms the variability in distribution and number of gastrin-secreting (G) cells (Solcia, Vassallo, and Capella, 1969) observed by morphological, cytochemical, and electron microscopical studies. In cases with presumed secondary hypersecretion a profound degree of G-cell hyperplasia is ob...

viewed as a signal transduction messenger. We present herein the cytochemical approach for detecting NADPH oxidase actMty in human neutrophils under both an electron microscope and a CCD camera-equipped fluorescence microscope, The applications of the cytochemical methodology are also ment;oned in this review. EspeciaHy. the following mechanisms for the release of superoxide from human neutroph...

[There are serious limitations on the available histochemical, biochemical, and physical methods for estimating precise quantities and locations of nucleic acids.] Reliable results can therefore only be obtained if all available tests are applied and give concordant results; it is also imperative, whenever possible, to check cytochemical results with microchemical estimations of pentoseand thym...

Senna occidentalis (L.) Link, subarbusto pertencente à família Fabaceae, conhecido popularmente como fedegoso, é uma espécie invasora com grande potencial biológico para uso medicinal. Este estudo objetivou avaliar o índice meiótico, viabilidade polínica e composição citoquímica dos grãos de pólen S. occidentalis. Botões florais 20 indivíduos fedegoso foram coletados no município Alta Floresta,...

The lead salt method introduced by Wachstein and Meisel (12) for the cytochemical demonstration of ATPase activity was modified and used to determine sites of activity on red cell ghost membranes. Preliminary studies showed that aldehyde fixation and standard concentrations of the capture reagent Pb(NO(3))(2) resulted in marked inhibition of the ATPase activity of these membranes. By lowering t...

Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formation. Cytochemical tests revealed enzyme reaction product on all mitochondrial me...