The cytochemical technique of Lee and Torack for the demonstration of aspartate aminotransferase activity was tested on a model system consisting of either total liver homogenate or the mitochondrial or soluble cytoplasmic fraction, incorporated in polyacrylamide film. After incubation of portions of film in a medium of alpha-ketoglutarate, L-aspartate, and lead nitrate, the lead oxaloacetate f...

Electron microscope cytochemical localization of glucose-6-phosphatase in the developing hepatocytes of fetal and newborn rats indicates that the enzyme appears simultaneously in all the rough endoplasmic reticulum of a cell, although asynchronously within the hepatocyte population as a whole. To confirm that the pattern of cytochemical deposits reflects the actual distribution of enzyme sites,...

Electron microscope cytochemical localization of glucose-6-phosphatase in the developing hepatocytes of fetal and newborn rats indicates that the enzyme appears simultaneously in all the rough endoplasmic reticulum of a cell, although asynchronously within the hepatocyte population as a whole. To confirm that the pattern of cytochemical deposits reflects the actual distribution of enzyme sites,...

IN THE IXVESTIGATION of neuromuscular disease, histologic examination of skeletal muscle biopsies is generally accepted as an important part both of routine diagnostic procedures and of more specialized research. The purpose of the present report is to establish that the use of histoand cytochemical technics combined with light microscopy is also an essential aspect of both types of investigati...

background: information regarding serum biochemicalprofile can reflect cardiovascular performance in animals.although studies have evaluated the inter-relationship amongcardiovascular biomarkers in animals and human beings, thereare no reports of such a probable relationship in camelids.objectives: the aim of the present study was to provide dataon the correlations among cardiovascular biomarke...

Knowledge of the mechanisms regulating the sequence of changes whereby neuroblasts differentiate into neurons is fundamental to an understanding of how reflex arcs and higher association pathways become organized. The manner and extent to which the development of neurons is regulated by intra-cellular, presumably genetic, mechanisms and through the interaction between cells, either those of the...