BACKGROUND Embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. This process may affect the viability and developmental potential of embryos. OBJECTIVE In this study, the effect of the vitrification cryotop method on the expression level of Oct4 and Mest developmental genes in mouse blastocyst...

background: the risk of multiple pregnancies often present in programs of in vitro fertilization (ivf) is an important force for embryo cryopreservation. on the other hand ethical restriction and assurance of potential fertility following chemo/radio therapy has led scientists to focus on female gamete preservation. objective: optimizing vitrification protocol by using less concentrated cryopro...

Background: The aim of this study was to compare two vitrification systems (Cryotop and OPS) on mouse MII oocytes and evaluate its effects on expression of heat shock protein A1A and MnSOD genes. Materials and Methods: Groups of about 15 oocytes without cumulus complexes (granulose, et. al.) were collected and exposed to equilibration and vitrification solutions before loading the oocytes into ...

background: an evaluation of the developmental competence of vitrified mouse germinal vesicle (gv) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. materials and methods: mouse gv oocytes were considered in cumulus-denuded oocytes (cdos) and cumulus-oocyte complexes (coc...

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This study was conducted to compare the efficiency of four vitrification methods: straws in goblet, straws in Hasler device, cryloop and cryotop methods on blastocyst viability and the extent of apoptosis using the TUNEL technique in bovine embryos. Blastocysts were produced in vitro by standard procedures. The base medium for vitrification was Syngro holding medium. Embryos were vitrified by t...

Survival and development of human embryos was compared following slow cooling versus vitrification involving more than 13,000 vitrified embryos. In addition, the efficacy of an open system, the Cryotop, and a closed vitrification system, the CryoTip(trade mark), were compared using human blastocysts. One hundred percent of vitrified human pronuclear stage embryos survived and 52% developed to b...

BACKGROUND Oocyte cryopreservation is an important part of modern fertility treatment. The effect of vitrification on the fertilization and developmental rates of embryo is still a matter of debate. OBJECTIVES This study aimed to investigate the effect of vitrification on the success of mouse oocyte maturation, fertilization, and preimplantation development in vitro. MATERIALS AND METHODS I...