This paper presents results pertaining to the numerical modelling of the cryogenic system at the Canadian Light Source. The cryogenic system consists of a cryostat that houses a Radio Frequency (RF) cavity used for boosting the energy of an electron beam. For consistent operation of the RF cavity, it must be kept immersed in liquid helium at a constant level with the pressure in the gas space m...

Cryostat sections of unfixed human breast carcinomas were assayed for oestrogen receptor (ER) content using an isoelectric focusing method to separate the receptor-bound oestradiol. Adjacent sections from the same tissue block were stained so that the tumour content could be estimated by point counting and the ER concentration adjusted to compensate for variations in cellularity. Elastosis was ...

Biotin-avidin systems can be used as an alternative to indirect antibody sandwich methods in the detection of T lymphocyte subsets in cryostat sections of human lymphoid tissue. Appreciable endogenous avidin binding activity (EABA) has been found, however, in human lymph nodes and tonsils. Such EABA can be a source of false positive staining when biotin-avidin detection systems are used to iden...

The applications and advantages of the cryostat are receiving increasing recognition for the preparation of fresh frozen sections for histochemistry, fluorescence microscopy, and surgical or autopsy pathology.' " With the cryostat, it is possible to prepare thin unwrinkled sections of single or multiple pieces of fresh tissue. Tissues that are very difficult to cut by means of the conventional ...

We report a photoluminescence imaging system for locating single quantum emitters with respect to alignment features. Samples are interrogated in a 4 K closed-cycle cryostat by a high numerical aperture (NA = 0.9, 100× magnification) objective that sits within the cryostat, enabling high efficiency collection of emitted photons without image distortions due to the cryostat windows. The location...