S Introduction ince Whittingham et al. first succeeded in cryopreserving mouse embryos in 1972, there has been remarkable progress in both basic and applied research enabling us to preserve the embryos of at least 13 more mammalian species. Moreover, every process used for freezing and thawing embryos has been refined and extremely simplified. Specifically: 1) The time required for the embryo t...

The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate iPS cells with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. GIBCO media and reagents have been at the forefront of pluripotent stem cell research for years. Knockout DMEM supplemented with Kn...

a:4:{s:10:"Background";s:2314:"The research made on stem cells through the last decade and following that the great achievements made applying stem cells in treating vast number of diseases have turned the issue of Cord Blood collecting and saving (cryopreserving) to a critical one. A prompt demand and access to stem cells and applying them in treating a wide range of terminal diseases have led...

The roe deer is a monoestrous species with very short rutting season. present work reports the most suitable period for collecting epididymal sperm and describes effect of two cooling rates on post-thaw quality sperm. Testes were collected 24–48 h after death. Samples flushed from epididymis subjected to either (1) dilution in Tris-citric acid-glucose-egg yolk-based medium glycerol, slow freezi...

Abstract The regulation of surrogacy in South Africa centres on a scheme judicial confirmation agreements before the start surrogate pregnancy. If such is granted by court, actions taken execution agreement are lawful, and itself enforceable. Against this background, question has arisen: it lawful to perform vitro fertilisation (IVF) agreement? This salient question: In circumstances where egg ...

Microsurgical epididymal sperm aspiration was a great advance in the therapy of patients with non-reconstructable, obstructive azoospermia, most notably congenital bilateral absence of the vas deferens. Using conventional in-vitro fertilization, pregnancies were rarely achieved because the rate of oocyte fertilization was extremely poor. However, the use of retrieved spermatozoa in conjunction ...

Articular cartilage and the menisci of the knee joint lack intrinsic repair capacity; thus, injuries to these tissues result in eventual osteoarthrotic changes to the joint. Tissue engineering offers the potential to replace damaged cartilage and mitigate long-term debilitating changes to the joint. In an attempt to enhance the ability of adult articular chondrocytes (ACs) and meniscus cells (M...

Dientamoeba fragilis is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the in vitro cultivation and cryopreservation of Dientamoeba has meant that few studies have been conducted to investigate its biology. The objective of this study was to define, for the first time, in vitro culture conditions able to support the long-term in vit...

Procedures for cryopreserving embryos vary considerably, each having its specific advantages and disadvantages in terms of technical feasibility, embryo survival yield, temperature permissibility and species- or strain-dependent applicability. Here we report a high osmolality vitrification (HOV) method that is advantageous in these respects. Cryopreservation by vitrification is generally very s...