Cryo-electron microscopy has been used exclusively so far for single particle analysis or crystallography of purified proteins. Recently, technical improvements on rapid freezing and cryo-microtome permitted structural analysis of organelle or macromolecules in native state using whole cells (1,2) and vitreous cryo-sections (3). However, cryo-sections don’t scatter electrons so much and therefo...

Today, cryo-electron tomography (cryo-ET) is the only technique that can provide near-atomic resolution structural data on macromolecular complexes in situ. Owing to strong interaction of an electron with matter, high-resolution cryo-ET studies are limited specimens a thickness less than 200 nm, which restricts applicability peripheral regions cell. A complex workflow comprises preparation thin...

Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electro...

The traditional tools of the structural biologist seeking to understand macro-molecules and their complexes are X-ray crystallography and NMR spectroscopy. Singleparticle cryo electron microscopy (“cryo-EM”) has established itself as a new structural biology technique over the last 15 years. Spectacular insights into the functioning of macromolecular complexes have been achieved especially from...

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...

BACKGROUND Transbronchial lung biopsy with flexible forceps is the most commonly used technique in diagnosis of diseases diffusely involving the lung parenchyma. Recently, transbronchial lung biopsy using the flexible cryoprobe (cryo-transbronchial lung biopsy) has also been reported. Herein, we perform a systematic review and meta-analysis describing the efficacy and safety of cryo-transbronch...

Cryo-electron microscopy (cryo-EM) is a microscopy technique used to discover the 3D structure of molecules from very noisy 2D images. This thesis presents two projects that use algebra to describe cryo-EM datasets. In the first part, we obtain an algebraic description of common lines datasets. Common lines are lines of intersection between cryo-EM images in 3D. They are an important ingredient...

The patient was a 39-year-old female with recurrent paroxysmal, regular narrow QRS complex tachycardia. Atrioventricular nodal reentrant tachycardia (AVNRT) was induced. The cryo-ablation attempts (-80°C, 240 second) were performed in the inferior-posterior triangle of Koch. We observed several junctional beats during cryo-ablation. After successful cryo-ablation, AVNRT induction was repeatedly...

Cryo-electron tomography (cryo-ET) is currently the only method available through which pleomorphic biological specimens are imaged to produce three-dimensional (3D) structures at ~2 to 10 nm resolution [1]. There remain challenges associated with imaging intact bacteria and pleomorphic viruses by cryo-ET. In particular, investigators cope with the low contrast of cryo-preserved, unstained spec...

In this study, the effect of heat shock on frozen-thawed blastocysts was evaluated using in vitro-produced (IVP) bovine embryos. In experiment 1, the effects of 6 h of heat shock at 41.0 C on fresh blastocysts were evaluated. HSPA1A expression as a reflection of stress was increased by heat shock (P < 0.05), but the expressions of the quality markers IFNT and POU5F1 were not affected. In experi...