INTRODUCTION: The feasibility of using crosslinking reagent, e.g., the genipin or methylglyoxal, to enhance the biomechanical functions of the injured or degenerated intervertebral disc has been extensively investigated. Our previous study found that genipin could recover the disc dynamic properties of mild denatured disc; however, the recovery efficiency was degraded by fatigue loading. Genipi...

Photo-initiated polymer networks were formed by copolymerization of tert-butyl acrylate with di(ethylene glycol) dimethacrylate (DEGDMA) or poly(ethylene glycol) dimethacrylate (PEGDMA). The degree of crosslinking was systematically varied by modifying the weight fraction and molecular weight of the dimethacrylate crosslinking agent. An increase in effective crosslink density with increasing cr...

β-Nitroalcohols (βNAs) are promising corneoscleral crosslinking agents for the treatment of diseases such as keratoconus and myopia. Although it is believed that formaldehyde is released from the crosslinking reactions of βNAs, the mechanism by which βNAs react with amine-functionalized polymers has yet to be known. In this study, we present the reaction mechanism of the βNA crosslinking. Fouri...

UV laser crosslinking is a potentially powerful tool to investigate transient DNA-protein interactions and binding kinetics in intact cells. As the processes underlying UV laser crosslinking are not fully understood, we have performed a study of the influence of laser pulses with different physical parameters on crosslinking of the progesterone receptor to an oligonucleotide containing a hormon...

There is a general interest among paper and board makers to produce lighter and bulkier paper and board products. The aim of the present study was to characterize chemically crosslinked hardwood kraft fibers regarding their potential in making bulky paper and board. Crosslinking agents are molecules that contain two or more reactive ends that form inter-chain covalent bonds with cellulosic hydr...

Crosslinking and the resultant changes in mechanical properties have been shown to influence cellular activity within collagen biomaterials. With this in mind, we sought to determine the effects of crosslinking on both the compressive modulus of collagen-glycosaminoglycan scaffolds and the activity of osteoblasts seeded within them. Dehydrothermal, 1-ethyl-3-3-dimethyl aminopropyl carbodiimide ...

Incubation in HeLa nuclear extract of a ^P-labeled 61 nucleotlde-tong RNA corresponding to the lariat branch site/polypyrlmldlne tract/3' splice site of the first Intron of human /S-globin pre-mRNA led to the crosslinking of a single protein of -62,000 mol. wt. (p62). p62 corresponds to a polypyrimidine tract-binding protein recently described by Garcia-Blanco et al. (Genes & Dev. 3: 1874-1886,...

Though silane chemistry has been used to crosslink unfilled polyethylene for many years, such crosslinking has only been recently applied to wood plastic composites to improve properties such as creep resistance. However, the presence of wood significantly changes the silane chemistry and a greater understanding is necessary for optimal processing and performance. We investigated three differen...

A novel crosslinking agent, suberoyl chloride, was used to crosslink N-phthaloyl acylated chitosan and improves the properties of chitosan membranes. Membranes with different crosslinking degrees were synthesized. The derivatives were characterized by Fourier transform infrared spectroscopy and ¹³C solid state nuclear magnetic resonance spectroscopy, which indicated that the crosslinking degree...