To estimate the ultimate extent of myocardial damage during evolving myocardial infarction in conscious dogs and patients, we analyzed early serum creatine phosphokinase (CPK) changes with nonlinear curve-fitting techniques. In experiments with dogs, serial serum CPK changes were fit to a log-normal function by the least squares method; the extent of the completed infarct was calculated by anal...

A radioimmunoassay was developed to measure serum levels of the B isoenzyme of creatine kinase(ATP: creatine N-phosphotransferase, EC 2.7.3.2) (CPK) in order to evaluate the time course and frequency of MB isoenzyme elevation in patients with acute myocardial infarction. The method can identify as little as 0.2 ng of the B portion of the CPK-MB isoenzyme, does not significantly crossreact with ...

Autosomal recessive polycystic kidney disease (ARPKD) is a rare but devastating inherited disease in humans. Various strains of mice that are homozygous for the cpk gene display renal pathology similar to that seen in human ARPKD. The PKD progresses to renal insufficiency, azotemia, and ultimately a uremic death by approximately 3 wk of age. This study characterizes PKD in mice that are homozyg...

or discontinue AZA/MTX had been made at consultant level, despite previous reports that transaminase elevations may be found in myositis [2–4] and other muscle diseases [4–6]. Transaminases are important intracellular components of metabolic function in many cells, including those of muscle, and elevated ALT levels may reflect transaminase leakage into the bloodstream, due to myofibrillar damag...

Creatine Phosphokinase-Linked Immunoglobulin in a Patient with Hypokalemic Myopathy Dr. H. Arai, Department of Neurology, Motojima General Hospital, Ota, Gunma 373 (Japan) A macromolecular complex between creatine phosphokinase (CPK) and an immunoglobulin [1], termed macro-CPK type 1 [2, 3], is most likely an immune complex [4, 5] and has been identified in a variety of diseases including ische...

Since creatine phosphokinase (CPK) is found predominantly in myocardial and skeletal muscle cells, in contrast to cells participating in the inflammatory response, it was considered likely that measurement of activity of this enzyme in the heart would provide a sensitive and relatively specific index of the extent of ischemic injury following acute coronary artery occlusion. Accordingly, CPK ac...

The clinical and laboratory applications of an assay method for serum creatine phosphokinase (CPK) utilizing a reagent capsule were investigated. Continuous recording of the change in absorbance at 340 mu, during the assay period gave a slightly sigmoid curve. The time interval between serum addition and the beginning of the linear portion of the absorbancecurve varied with the level of enzyme ...

Using an antibody specific for creatine phosphokinase (CPK), we have discovered an association between that enzyme and the cytoskeleton. Immunofluorescence observations show that CPK is associated with intermediate filaments in PTK cells and BALB/3T3 cells. The CPK distribution also follows intermediate filaments when cells are treated with colchicine.