Liquid chromatography (LC) coupled with mass spectrometry (MS) detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, and rapid method development. In this paper, a new liquid chromatography-tandem mass spectrometry (LC-...

A method for simultaneous determination of nine sulfonylurea herbicides in water and soil samples with solvent demulsification-dispersive liquid-liquid microextraction based on solidification of floating organic drop coupled with high perfomence liquid chromatography was established. 120 μL of 1-dodecanol as extractant and 400 μL of methanol as dispersing reagent were injected into the sample s...

This review describes analytical methods developed for the separation, identification and quantitation of trans-isomers of unsaturated fatty acids. Although these methods are applicable to a wide variety of food matrices, we have particularly emphasised the analysis of trans unsaturated fatty acids in milk fat. Various methods are described such as infrared spectroscopy, direct gas chromatograp...

Recently, H. Kasai reported an automatic, precise method of 8-hydroxydeoxyguanosine (8-OH-dG) analysis in urine by high performance liquid chromatography coupled to an electrochemical detector (HPLC-ECD). It is based on a cleaning-up step by anion-exchange chromatography and a further purification step using reverse phase chromatography before detection, by the ECD. In this communication, we re...

novel dispersive liquid-liquid microextraction (dllme), coupled with high performance liquid chromatography with photodiode array detection (hplc-dad) has been applied for the extraction and determination of cyproheptadine (cph), an antihistamine, in human urine samples. in this method, 0.6 ml of acetonitrile (disperser solvent) containing 30 µl of carbon tetrachloride (extraction solvent) was ...

Cloud point extraction (CPE) as a rapid, simple and efficient method coupled with high performance liquid chromatography (HPLC) was used for sample preparation and subsequent determination of carbazole, trinitrocarbazole (TrNC) and tetra nitro carbazole (TNC) in water samples. Some effective parameters on extraction, such as volume of Triton X-100, extraction time, extraction temperature, ionic...

Potential of ultra-performance liquid chromatography (UPLC) separation strategy coupled with tandem (in space) mass spectrometric detection (MS/MS) in multiresidue pesticide analysis was critically assessed. Performance parameters such as number of theoretical plates, height of theoretical plate, peak symmetry and peak capacity were measured/calculated on the basis of data generated by analysis...

Objective: Establish a quick, precise and specific method to determine whether the moutan cortex obtained from market is processed with sulfur; provide a reliable method for the scientific evaluation. Methods: Three methods, including acid-base titration method, high-performance liquid-chromatography (HPLC) method, and ultraperformance liquid chromatography coupled with time-of-flight mass spec...

Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the foll...

Liquid chromatography electrospray ionization mass spectrometry is a very powerful technique that combines the liquid chromatography separation with highly selective and sensitive mass detection, but the ionization process is susceptible to matrix signal suppression. Signal suppression, therefore, presents a challenge in doing quantitative liquid chromatography/mass spectrometry applications. U...