نتایج جستجو برای: cotyledonary node

تعداد نتایج: 170755  

Journal: :Pesquisa Agropecuária Tropical 2021

ABSTRACT Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of genus. Tissue culture pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate morphogenesis P. green and purple varieties, view massive propagation species. The morphogenic capacity explants cotyledonary no...

2012
Sushil Kumar Yadav Sweety Katikala Varalaxmi Yellisetty Annapurna Kannepalle Jyothi Lakshmi Narayana Vanaja Maddi Maheswari Mandapaka Arun Kumar Shanker Venkateswarlu Bandi Kirti Pulugurtha Bharadwaja

A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...

Journal: :journal of agricultural science and technology 2014
s. c. liu g. c. zhang l. f. yang m. mii j. y. gai

a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

Journal: :Plant & cell physiology 2005
Cinzia Solfanelli Francesca Ceron Fabio Paolicchi Lucia Giorgetti Chiara Geri Nello Ceccarelli Yuji Kamiya Piero Picciarelli

We have isolated PcGA3ox1, a cDNA clone from developing runner bean (Phaseolus coccineus) seeds that shows significant amino acid homology with the gibberellin (GA) 3-oxidases. A recombinant fusion protein of PcGA3ox1 converted GA20 and GA9 to GA1 and GA4, respectively. In situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-st...

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