Cell-based therapies have been increasingly explored as a potential alternative treatment method of corneal endothelial dysfunction. Up to date, researchers have mainly dedicated enormous efforts to establishing fundamental techniques for culture and characterization of human corneal endothelial cells (HCECs) and human corneal endothelial progenitor cells (HCEPCs). However, in terms of cell del...

PURPOSE To study the effects of long-term contact lens wear on morphologic and physiologic properties of corneal endothelial cells. METHODS The endothelial permeability to fluorescein and the rate of corneal deswelling from hypoxia-induced edema were measured in 20 long-term (mean, 17+/-9 years; range, 5-33 years) contact lens wearers and 20 age-matched control subjects. From these data, the ...

AIM To investigate the corneal endothelial cell density and morphology and central corneal thickness in the Guangxi Maonan and Han adolescent students of China. METHODS Noncontact specular microscope (Topcon SP3000P, Tokyo, Japan) was performed in 133 adolescent students of Maonan nationality (M:F 54:79) and 105 adolescent students of Han nationality (M:F 50:55), 5 to 20y of age, who were ran...

Extracellular matrices (ECM) isolated from chick embryo fibroblast and human and rabbit corneal stromal cells induce polarization and elongation of corneal endothelial cells in culture. ECM isolated from rabbit corneal or bovine aortic endothelial cells neither polarize nor elongate corneal endothelial cells in culture. By indirect immunofluorescence, fibronectin is seen as arrays of long fiber...

OBJECTIVE To establish a reproducible method for the culture of primary equine corneal epithelial cells, keratocytes, and endothelial cells and to describe each cell's morphologic characteristics, immunocytochemical staining properties and conditions required for cryopreservation. PROCEDURES Corneas from eight horses recently euthanized for reasons unrelated to this study were collected asept...

PURPOSE In Asian countries, laser iridotomy for the treatment of angle-closure glaucoma is a common cause of bullous keratopathy, which may be associated with a shallow anterior chamber and dark iris pigmentation in Asians. Several cases of corneal decompensation after argon laser iridotomy have been reported. In the present study, we evaluated the harmful effects of argon laser iridotomy on th...

PURPOSE To investigate the effect and safety of a selective Rho kinase inhibitor, ripasudil 0.4% eye drops, on corneal endothelial cells of healthy subjects. DESIGN Prospective, interventional case series. METHODS In this study, 6 healthy subjects were administered ripasudil 0.4% in the right eye twice daily for 1 week. Morphological changes and corneal endothelial cell density were examine...

PURPOSE To analyze the corneal endothelial cell density in healthy adult emmetropic subjects. METHODS We analyzed the corneal endothelial cell density of a group made up of 225 emmetropic subjects (n=225). As age-matched control groups we analyzed two other groups, one made up of myopic subjects (n=209) and the other made up of hyperopic subjects (n=203). We recorded the mean of three consecu...

Citation: Zhang W, Ogando DG, Kim ET, et al. Conditionally immortal Slc4a11 / mouse corneal endothelial cell line recapitulates disrupted glutaminolysis seen in Slc4a11 / mouse model. Invest Ophthalmol Vis Sci. 2017;58:3723–3731. DOI:10.1167/ iovs.17-21781 PURPOSE. To establish conditionally immortal mouse corneal endothelial cell lines with genetically matched Slc4a11þ/þ and Slc4a11 / mice as ...