The binding reaction between carcinoembryonic antigen (CEA) and concanavalin A (Con A) has been studied. CEA, prepared from liver métastasesin patients with carcinoma of the colon, was tested against Con A for precipitation. By using the techniques of immunodiffusion and counlerelectrophoresis, a precipitate line between CEA and Con A was observed, which was different from that seen between ei...

Concanavalin A (Con A) concentration dependence of agglutinability of a mutant of Rous sarcoma virus which is temperature-sensitive for transformation was examined. Con A agglutinability of the virion was quantitatively expressed by measuring radioactivities of 60 to 70 S RNA extracted from Con A-agglutinated material. The mutant grown at a permissive temperature (35 degrees C) agglutinated at ...

We have demonstrated that stable Concanavalin A (Con A)-coated supramolecular nanofibers can be constructed by complexation of self-assembled mannose nanofibers with Con A. The extended multivalent Con A nanofibers bind strongly to T cell surfaces and promote T cell activation.

Human placenta alkaline phosphatase (HP-ALP), a glycoprotein, was stained histochemically for the purpose of examining the concanavalin A (Con A) binding sites on the cell surface. HP-ALP was bound to the cell surface by Con A. This simple method successfully detected Con A binding sites on the cell surface.

cultured lymphoblasts required physical contact with responding lymphocytes to induce transformiiation. In quantitative terms, lymphocytes incorporate as much thymidine when,mixed with irradiated cultured lymphoblasts as they (lo in response to l)hytolhenma-glutin1in. The stimulation of lymphocyte transformation by allogeneic cultured lymphoblasts did not parallel the stimulation of lymphocyte ...

Concanamycins A, B and C were isolated from the mycelium of Streptomyces diastatochromogenes S-45 as effective inhibitors of the proliferation of mouse splenic lymphocytes stimulated by concanavalin A. They represent a new class of 18-membered macrolide antibiotics, and are biologically active in vitro against several fungi and yeasts, but not against bacteria. Concanamycin A, the main componen...

Peritoneal macrophages from mice injected 4 days previously with Brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (HRP)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng HRP/100 mug cell protein/h. Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate leve...

Interleukin (IL)-17 is exclusively produced by CD4 helper T-cells upon activation. It most often acts as a pro-inflammatory cytokine, which stimulates the release of pro-inflammatory cytokines IL-6, IL-8, TNF-α, and granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, we studied the in-vitro IL-17 response to specific antigens and a variety of mitogens and compared the IL-1...